Recombinant Human G-CSFR/CD114 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human G-CSFR/CD114 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the G-CSF-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 1-4 µg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF.
• Source: Mouse myeloma cell line, NS0-derived human G-CSF R/CD114 protein
  Glu25-Pro621
• Accession #: Q99062
• N-terminal Sequence: Glu25
• Protein/Peptide Type: Recombinant Proteins
• Gene: CSF3R
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human G-CSFR/CD114 Protein, CF

• CD114 antigen
• CD114
• colony stimulating factor 3 receptor (granulocyte)
• CSF3R
• Csfgr
• G-CSF R
• G-CSF receptor
• GCSFR
• G-CSFR
• GCSFRG-CSF-R
• granulocyte colony-stimulating factor receptor

Background

Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 836 amino acids (aa) in length, with 24 amino acid signal sequence, a 603 aa extracellular domain, a 23 aa transmembrane domain, and a 186 aa cytoplasmic domain. As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C-terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor. The existence of 80 & 85 kDa forms of soluble G-CSF R in human serum has been reported (2). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (3). Human and mouse G-CSF R have a homology of 62.5%.

G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1).

1. Nicola, N.A., in Cytokine Reference, (2001) Oppenhiem, J.J. and M. Feldmann, eds. Academic Press p. 193
2. Iwasaki, H. et al. (1999) J. Immunol. 163:6907.
3. Mitsui, T. et al. (2003) Blood. 101:2990.

Publications for G-CSFR/CD114 (381-GR/CF)(2)

Publications using 381-GR/CF

• J Skokowa, B Hernandez, M Coles, M Ritter, M Nasri, J Haaf, N Aghaallaei, Y Xu, P Mir, AC Krahl, KW Rogers, K Maksymenko, B Bajoghli, K Welte, AN Lupas, P Müller, M ElGamacy A topological refactoring design strategy yields highly stable granulopoietic proteins Nature Communications, 2022-05-26;13(1):2948. 2022-05-26 [PMID: 35618709] (Surface Plasmon Resonance, Human)
• S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling J. Biol. Chem, 2017-03-09;0(0):. 2017-03-09 [PMID: 28280243] (Bioassay)

Bioinformatics

• Gene Symbol: CSF3R
• Uniprot:
  • Q99062(Human)