Recombinant Human G-CSF Protein


Recombinant Human G-CSF (Catalog # 214-CS) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 10-60 pg/mL.
1 μg/lane of Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18.5 kDa.

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Recombinant Human G-CSF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
E. coli-derived human G-CSF protein
Thr31-Pro204, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17-19 kDa, reducing conditions
Read Publications using
214-CS in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human G-CSF Protein

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • Pluripoietin


G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma . Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

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Publications for G-CSF (214-CS)(53)

We have publications tested in 3 confirmed species: Human, Mouse,  Human.

We have publications tested in 6 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo, SDS-Page.

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Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using 214-CS Applications Species
Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F; c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities Cancers 2023-05-05 [PMID: 37174093] (Bioassay, Human) Bioassay  Human
JR James, J Curd, JC Ashworth, M Abuhantash, M Grundy, CH Seedhouse, KP Arkill, AJ Wright, CLR Merry, A Thompson Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML International Journal of Molecular Sciences, 2023-02-20;24(4):. 2023-02-20 [PMID: 36835644] (Bioassay, Human) Bioassay Human
CM Biggs, A Cordeiro-S, SV Prykhozhij, AP Deveau, Y Lin, KL Del Bel, F Orben, RJ Ragotte, A Saferali, S Mostafavi, L Dinh, D Dai, KG Weinacht, K Dobbs, L Ott de Bru, M Sharma, K Tsai, JJ Priatel, RA Schreiber, J Rozmus, MC Hosking, KE Shopsowitz, ML McKinnon, S Vercautere, M Seear, LD Notarangel, FC Lynn, JN Berman, SE Turvey Human JAK1 gain of function causes dysregulated myelopoeisis and severe allergic inflammation JCI Insight, 2022-12-22;7(24):. 2022-12-22 [PMID: 36546480] (Cell Culture, Human) Cell Culture Human
JH Kim, JH Hong, KW Kang, BH Lee, Y Park, BS Kim Generation of the human pluripotent stem cell lines KUMi005-A from a patients with multiple myeloma Stem Cell Research, 2022-10-13;65(0):102939. 2022-10-13 [PMID: 36332466] (Bioassay, Human) Bioassay Human
YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells Science Advances, 2022-08-24;8(34):eabn5522. 2022-08-24 [PMID: 36001668] (Bioassay, Human) Bioassay Human
JH Kim, KW Kang, BH Lee, Y Park, BS Kim Generation of human induced iPSC KUMi003-A from acute promyelocytic leukemia (APL) M3 Oncogene, 2022-07-09;63(0):102861. 2022-07-09 [PMID: 35905670] (Bioassay, Human) Bioassay Human
BA Krishna, AB Wass, EA Murphy, CM O'Connor Design of a US28 ORF Deletion Virus in a Temperature-Sensitive Cytomegalovirus Strain Fails to Promote Lytic Replication in Hematopoietic Cells Viruses, 2022-06-12;14(6):. 2022-06-12 [PMID: 35746751] (Bioassay, Human) Bioassay Human
JH Hong, JH Kim, KW Kang, BH Lee, Y Park, BS Kim Generation of a human induced pluripotent stem cell line KUMi006 from a patient with multiple myeloma Stem Cell Research, 2022-03-28;61(0):102767. 2022-03-28 [PMID: 35397398] (Bioassay, Human) Bioassay Human
JH Kim, KW Kang, BH Lee, Y Park, H Jung Sung, BS Kim A human pluripotent stem cell line KUMi004-A generated from a patient with chronic lymphocytic leukemia Stem Cell Research, 2022-01-15;60(0):102668. 2022-01-15 [PMID: 35066240] (Bioassay, Human) Bioassay Human
JH Kim, KW Kang, BH Lee, Y Park, BS Kim Generation of induced pluripotent stem cell line KUMi002-A with normal karyotype from a patient with Philadelphia chromosome-positive chronic myeloid leukemia Stem Cell Research, 2021-07-12;55(0):102465. 2021-07-12 [PMID: 34303284] (Bioassay, Human) Bioassay Human
Show All 53 Publications.

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Gene Symbol CSF3