Recombinant Human Fibronectin Fragment 3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Fibronectin Fragment 3 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. When 5 x 104 cells/well are added to rhFibronectin Fragment 3/amino acids
1266-1908 coated plates (1 µg/mL with 100 µL/well), approximately 50‑75% will adhere after 30 minutes at 37 °C.
Optimal concentration depends on cell type as well as the application or research objective.
Source
Mouse myeloma cell line, NS0-derived human Fibronectin protein
Glu1266-Pro1908, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu1266
Protein/Peptide Type
Recombinant Proteins
Gene
FN1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
70.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-90 kDa, reducing conditions
Publications
Read Publication using
3938-FN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fibronectin Fragment 3 Protein, CF

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin (FN) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matrix (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids (1, 2). The protein subunit is made up of three types of homologous structural motifs termed FN type I, type II, and type III repeats (3 - 5). Alternative splicing generates multiple isoforms of Fibronectin which may have insertions of extra type III domains (EDA and EDB) or alteration of the type III connecting segment (IIICS) (5). Fibronectin is a ligand for many molecules including fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes such as cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of soluble Fibronectin to Fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization (1). FN1.3 includes the EDB plus type III domains #8 - 13 and the initial region of domain 14. These domains facilitate association with heparin, myocilin, ApoA, and integrins alpha 5 beta 1 and alpha V beta 3 (6 - 10). The tilt angle between type III domains #9 and #10 (which contains an RGD motif) determines the affinity of interaction with these integrins, suggesting how structural differences between fibrillar and soluble Fibronectin may influence their function (10). Within FN1.3, human Fibronectin shares 97% and 92% aa sequence identity with mouse and rat Fibronectin, respectively.

  1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
  2. Potts, J.R. and I.D. Campbell (1996) Matrix Biol. 15:313.
  3. Bernard, M.P. et al. (1985) Biochemistry 24:2698.
  4. Kornblihtt, A.R. et al. (1983) Proc. Natl. Acad. Sci. USA 80:3218.
  5. Kornblihtt, A.R. et al. (1985) EMBO J. 4:1755.
  6. Peters, D.M. et al. (2005) Exp. Cell Res. 303:218.
  7. Edelstein, C. et al. (2005) J. Lipid Res. 46:2673.
  8. Pytela, R. et al. (1985) Cell 40:191.
  9. Wayner, E.A. et al. (1989) J. Cell Biol. 109:1321.
  10. Altroff, H. et al. (2004) J. Biol. Chem. 279:55995.

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3938-FN
Species: Hu
Applications: Bioactivity

Publications for Fibronectin (3938-FN)(1)

We have publications tested in 1 confirmed species: Bacteria.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol FN1
Uniprot