Recombinant Human Fibronectin Fragment 2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Fibronectin Fragment 2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells.

  The ED₅₀ for this effect is 0.25-1.25 μg/mL.

  Optimal dilutions should be determined by each laboratory for each application.

• Source: Mouse myeloma cell line, NS0-derived human Fibronectin protein
  Ser607-Pro1265, with a C-terminal 6-His tag
• Accession #: NP_997647
• N-terminal Sequence: Ser607
• Protein/Peptide Type: Recombinant Proteins
• Gene: FN1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

Tween is a registered trademark of ICI Americas.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fibronectin Fragment 2 Protein, CF

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Fibronectin, encoded by the FN1 gene, is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matrix (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. The protein subunit is made up of three types of homologous structural repeats termed FN type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of the non-functional soluble fibronectin to fibronectin fibrils in the ECM is tightly regulated. The process is initiated by binding of fibronectin to cell surface integrins, resulting in conformational changes and exposure of cryptic epitopes necessary for polymerization (1, 2).

The recombinant human FN1.2 contains the first 7 FN type III domains (III1 - 7). III1 binds weakly to heparin and is thought to be important for self association and fibril formation. The recombinant human FN1.2 does not contain the known tripeptide RGD domain.

1. Johansson, S. et al. (1997) Frontiers in Bioscience 2:126.
2. Geiger, B. et al. (2001) Nature Reviews Molecular Cell Biology 2:793.

Bioinformatics

• Gene Symbol: FN1
• Uniprot:
  • NP_997647()