Recombinant Human FGFR4 Fc Chimera Protein, CF

Images

 
There are currently no images for FGFR4 (685-FR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human FGFR4 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 2‑6 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human FGF R4 protein
Human FGF R4
(Leu22-Asp369)
Accession # P22455
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FGFR4
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
65 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-110 kDa, reducing conditions
Publications
Read Publications using
685-FR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGFR4 Fc Chimera Protein, CF

  • CD334 antigen
  • CD334
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R4
  • FGFR4
  • FGFR-4
  • fibroblast growth factor receptor 4
  • JTK2hydroxyaryl-protein kinase
  • MGC20292
  • protein-tyrosine kinase
  • TKF
  • tyrosine kinase related to fibroblast growth factor receptor
  • tyrosylprotein kinase

Background

Fibroblast growth factor receptor 4 (FGF R4), also known as CD334, is a 110 kDa glycosylated transmembrane receptor tyrosine kinase (1). Mature human FGF R4 consists of a 348 amino acid (aa) extracellular domain (ECD) with three Ig‑like domains, a 21 aa transmembrane segment, and a 412 aa cytoplasmic domain that contains the tyrosine kinase domain (2). Within the ECD, human FGF R4 shares 90% and 88% aa sequence identity with mouse and rat FGF R4, respectively. Alternate splicing generates a potentially secreted isoform with a substitution encompassing the transmembrane segment (3). A 65 kDa N‑terminally truncated isoform lacks the signal peptide and first two Ig‑like domains. This isoform is produced in human pituitary adenomas and is constitutively phosphorylated and oncogenic (4). FGF R4 is widely expressed during embryonic development and in adult liver, kidney, and lung (5‑8). It binds FGF acidic, FGF basic, FGF‑8, -15, and -19 (2, 7, 9‑12). FGF R4 associates with beta-Klotho and sulfated glycosaminoglycans, and these interactions increase the affinity of FGF R4 for its ligands as well as its signaling capacity (8, 9, 12). FGF-19 induced signaling through FGF R4 is important for the regulation of bile acid synthesis and lipid and glucose homeostasis (10, 13). FGF R4 supports glucose tolerance and insulin sensitivity and protects against hyperlipidemia (13). It is down‑regulated in the liver during fasting and is up‑regulated by insulin (10). It can exert either proliferative or apoptotic effects on hepatocytes (8, 11). FGF R4 signaling is additionally required for skeletal muscle development in limbs (7, 14). FGF R4 interacts in cis with cell surface MMP-14, leading to down‑regulation of both proteins (15). In contrast, the Arg388 variant of FGF R4, which is associated with tumor progression in human cancer, is activated and stabilized by MMP-14 (15, 16).
  1. Haugsten, E.M. et al. (2010) Mol. Cancer Res. 8:1439.
  2. Partanen, J. et al. (1991) EMBO J. 10:1347.
  3. Takaishi, S. et al. (2000) Biochem. Biophys. Res. Commun. 267:658.
  4. Ezzat, S. et al. (2002) J. Clin. Invest. 109:69.
  5. Stark, K.L. et al. (1991) Development 113:641.
  6. Korhonen, J. et al. (1992) Int. J. Dev. Biol. 36:323.
  7. Marics, I. et al. (2002) Development 129:4559.
  8. Luo, Y. et al. (2010) J. Biol. Chem. 285:30069.
  9. Saxena, K. et al. (2010) J. Biol. Chem. 285:26628.
  10. Shin, D.J. and T.F. Osborne (2009) J. Biol. Chem. 284:11110.
  11. Wu, X. et al. (2010) J. Biol. Chem. 285:5165.
  12. Nakamura, M. et al. (2011) J. Biol. Chem. 286:26418.
  13. Huang, X. et al. (2007) Diabetes 56:2501.
  14. Kwiatkowski, B.A. et al. (2008) J. Cell Physiol. 215:803.
  15. Sugiyama, N. et al. (2010) Proc. Natl. Acad. Sci. 107:15786.
  16. Bange, J. et al. (2002) Cancer Res. 62:840.

Customers Who Viewed This Item Also Viewed...

NBP2-00932
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB600-1287
Species: Hu, Mu, Rt, Rb
Applications: WB, Flow, IHC, IP, CyTOF-ready
NBP2-66840
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
7460-F4/CF
Species: Hu
6829-F6/CF
Species: Hu
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB9120
Species: Hu
Applications: ICC
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF1280
Species: Mu
Applications: WB, Simple Western, IHC
NB400-153
Species: Hu, Mu
Applications: WB, ICC/IF
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC

Publications for FGFR4 (685-FR)(4)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 2 applications: Bioassay, ELISA Capture.


Filter By Application
Bioassay
(3)
ELISA Capture
(1)
All Applications
Filter By Species
Human
(2)
Chicken
(2)
All Species

Reviews for FGFR4 (685-FR) (0)

There are no reviews for FGFR4 (685-FR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for FGFR4 (685-FR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Recombinant Monoclonal Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human FGFR4 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FGFR4
Uniprot