Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein Summary

Additional Information
New rh Fas! HEK293 expressed; ~2 fold better activity; Lower endotoxin specification; No His tag!
Details of Functionality
Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED50 for this effect is 0.01‑0.04 µg/mL in the presence of 2 ng/mL recombinant human Fas Ligand.
Source
Mouse myeloma cell line, NS0-derived human Fas/TNFRSF6/CD95 protein
Human Fas
(Arg17-Asn173)
Accession # Q5T9P3
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Arg17 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FAS
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
45 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
326-FS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein

  • Apo-1 antigen
  • Apo-1
  • apoptosis antigen 1
  • Apoptosis-mediating surface antigen FAS
  • APT1
  • APT1FASTM
  • CD95 antigen
  • CD95
  • CD95ALPS1A
  • Fas (TNF receptor superfamily, member 6)
  • Fas AMA
  • Fas antigen
  • Fas
  • FAS1
  • FASLG receptor
  • TNFRSF6
  • TNFRSF6member 6
  • tumor necrosis factor receptor superfamily member 6

Background

Fas, also known as APO‑1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1-3). The 335 amino acid (aa) human Fas includes a 25 aa signal peptide, a 148 aa extracellular domain (ECD) with three cysteine-rich TNFR repeats, a 17 aa transmembrane sequence, and a 145 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature human Fas ECD shares 55%, 58%, 62%, 63%, and 64% aa sequence identity with mouse, rat, feline, bovine and porcine Fas, respectively. A human Fas isoform of 314 aa that lacks the transmembrane sequence is secreted by resting lymphocytes, while isoforms of 149, 132, 103 and 86 aa that also lack the DD and show substitutions for parts of the TNFR repeats are less prominently expressed (4-6). All appear to block the extrinsic apoptosis pathway induced by the Fas ligand (FasL, TNFSF6), a type II transmembrane protein of the TNF family that can be expressed on activated T‑lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2, 6). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death‑Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (7). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation‑induced death of T lymphocytes that terminates the immune reaction (1, 2, 8). In immune‑privileged tissues, infiltrating Fas‑bearing lymphocytes and inflammatory cells are killed by FasL engagement (9). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1-3). The Fas pathway also appears to cross‑communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).

  1. Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
  2. Strasser, A. et al. (2009) Immunity 30:180.
  3. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
  4. SwissProt accession P25445.
  5. Liu, C. et al. (1995) Biochem. J. 310:957.
  6. Papoff, G. et al. (1996) J. Immunol. 156:4622.
  7. Thorburn, A. (2003) Cellular Signaling 16:139.
  8. Barreiro, R. et al. (2004) J. Immunol. 173:1519.
  9. Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.

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326-FS
Species: Hu
Applications: Bioactivity

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Bioinformatics

Gene Symbol FAS
Uniprot