Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

Images

 
There are currently no images for Erythropoietin/EPO (287-TC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) Summary

Details of Functionality
Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.015-0.075 units/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin/EPO protein
Ala28-Arg193
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
EPO
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
37 kDa, reducing conditions
Publications
Read Publications using
287-TC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Instructions
Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of the EPO protein is required for biological activities in vivo (4). The mature human EPO protein shares 75% - 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce erythropoietin production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

  1. Koury, M.J. (2005) Exp. Hematol. 33:1263.
  2. Jacobs, K. et al. (1985) Nature 313:806.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

Customers Who Viewed This Item Also Viewed...

NBP1-19388
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
7270-IL/CF
Species: Hu
NBP2-59451
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-00622
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, TCS, KD, KO, LA
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow, KO
NLS3898
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
287-TC
Species: Hu
Applications: Bioactivity

Publications for Erythropoietin/EPO (287-TC)(38)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISA (Control).


Filter By Application
Bioassay
(31)
Cell Culture
(6)
ELISA (Control)
(1)
All Applications
Filter By Species
Human
(31)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using 287-TC Applications Species
JJ Montoya, MA Turnidge, DH Wai, AR Patel, DW Lee, V Gokhale, LH Hurley, RJ Arceci, C Wetmore, DO Azorsa In vitro activity of a G-quadruplex-stabilizing small molecule that synergizes with Navitoclax to induce cytotoxicity in acute myeloid leukemia cells BMC Cancer, 2019;19(1):1251. 2019 [PMID: 31881855] (Bioassay, Human) Bioassay Human
J Guan, X Liu, H Zhang, X Yang, Y Ma, Y Li, Z Gai, Y Liu Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49-50 exons in the DMD gene Stem Cell Res, 2019;42(0):101666. 2019 [PMID: 31812072] (Cell Culture, Human) Cell Culture Human
H Zhang, Y Ma, X Li, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu An integration-free iPSC line (SDQLCHi013-A) derived from a patient with maple syrup urine disease carrying compound heterozygote mutations in BCKDHA gene Stem Cell Res, 2019;41(0):101585. 2019 [PMID: 31775088] (Cell Culture, Human) Cell Culture Human
H Zhang, Y Ma, S Yu, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene Stem Cell Res, 2019;41(0):101611. 2019 [PMID: 31707209] (Cell Culture, Human) Cell Culture Human
J Guan, X Liu, H Zhang, Y Lv, X Wang, X Yang, Y Ma, Q Liu, Y Liu, W Sun Generation of an iPSC line (SDQLCHi015-A) from peripheral blood mononuclear cells of a patient with mental retardation type 15 carrying c.1007_1011del, p.(Ile336fs) in CUL4B gene Stem Cell Res, 2019;41(0):101628. 2019 [PMID: 31678776] (Cell Culture, Human) Cell Culture Human
Y Ma, H Zhang, S Zhang, R Dong, X Yang, Y Li, J Guan, Z Gai, Y Liu An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene Stem Cell Res, 2019;41(0):101577. 2019 [PMID: 31648101] (Cell Culture, Human) Cell Culture Human
Y Li, H Zhang, B Yan, Y Ma, X Yang, J Guan, Y Lv, M Gao, J Ma, Z Gai, Y Liu An induced pluripotent stem cell line (SDQLCHi006-A) derived from a patient with maple syrup urine disease type Ib carrying compound heterozygous mutations of p.R168C and p.T322I in BCKDHB gene Stem Cell Res, 2019;40(0):101579. 2019 [PMID: 31610500] (Cell Culture, Human) Cell Culture Human
Y Ma, H Zhang, X Yang, Y Li, J Guan, Y Lv, H Li, Y Liu, Z Gai Establishment of a human induced pluripotent stem cell line (SDQLCHi004-A) from a patient with nemaline myopathy-4 disease carrying heterozygous mutation in TPM2 gene Stem Cell Res, 2019;40(0):101559. 2019 [PMID: 31526942] (Bioassay, Human) Bioassay Human
FS Kittur, Y Lin, E Arthur, CY Hung, PA Li, DC Sane, J Xie Recombinant asialoerythropoetin protects HL-1 cardiomyocytes from injury via suppression of Mst1 activation Biochem Biophys Rep, 2019;17(0):157-168. 2019 [PMID: 30671548] (Bioassay, Mouse) Bioassay Mouse
L Cyganek, M Tiburcy, K Sekeres, K Gerstenber, H Bohnenberg, C Lenz, S Henze, M Stauske, G Salinas, WH Zimmermann, G Hasenfuss, K Guan Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes JCI Insight, 2018;3(12):. 2018 [PMID: 29925689] (Bioassay, Human) Bioassay Human
Show All 38 Publications.

Reviews for Erythropoietin/EPO (287-TC) (0)

There are no reviews for Erythropoietin/EPO (287-TC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Erythropoietin/EPO (287-TC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Erythropoietin/EPO Products

Bioinformatics Tool for Erythropoietin/EPO (287-TC)

Discover related pathways, diseases and genes to Erythropoietin/EPO (287-TC). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Erythropoietin/EPO (287-TC)

Discover more about diseases related to Erythropoietin/EPO (287-TC).
 

Pathways for Erythropoietin/EPO (287-TC)

View related products by pathway.

PTMs for Erythropoietin/EPO (287-TC)

Learn more about PTMs related to Erythropoietin/EPO (287-TC).

Blogs on Erythropoietin/EPO

There are no specific blogs for Erythropoietin/EPO, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) and receive a gift card or discount.

Bioinformatics

Gene Symbol EPO
Uniprot