Recombinant Human EGF-L6 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human EGF-L6 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Osada, A. et al. (2005) Exp. Cell Res. 303:148. The ED50 for this effect is 1-5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human EGF-L6 protein
Human EGF-L6
(Asn22-Arg363)
Accession # Q8IUX8
HP GGGSGGGSGGGS HHHHHH
N-terminus C-terminus
Accession #
N-terminal Sequence
Asn22
Protein/Peptide Type
Recombinant Proteins
Gene
EGFL6
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-55 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EGF-L6 Protein, CF

  • DKFZp564P2063
  • EGF repeat-containing protein 6
  • EGFL6
  • EGF-L6
  • EGF-like protein 6
  • EGF-like-domain, multiple 6
  • epidermal growth factor-like protein 6
  • MAEG
  • MAM and EGF domain containing
  • MAM and EGF domains-containing gene protein
  • W80

Background

Epidermal Growth Factor-like Domain, Multiple 6 (EGF-L6), also known as MAM- and EGF-containing (MAEG) is a member of the EGF repeat superfamily of proteins. This secreted protein contains five EGF-like repeats, an RGD motif that binds to Integrin alpha 8 beta 1, and a C-terminal MAM domain (1, 2). Within shared regions, human EGF-L6 shares 75% and 78% amino acid sequence identity with the mouse and rat orthologs, respectively. EGF-L6 is expressed in a multitude of fetal tissues during early development such as lung, heart, liver, spleen, cochlea, and placenta (3, 4). It is also expressed in the dermatome and dermatome-derived tissues including trunk dermis, hair follicles, and cranio-facial mesenchyme (5). EGF-L6 is expressed by osteoblastic-like cells and has been shown to act in a paracrine manner to promote migration of vascular endothelial cells, thus regulating angiogenesis in the local environment of the bone (4, 6). Additionally, EGF-L6 is expressed by epidermal stem cells in the hair follicle bulge and is believed to play a role in regulating the dermal vasculature (7). EGF-L6 is also produced by mature adipocytes and is thought to be involved in the process of adipose tissue expansion and the development of obesity (8). EGF-L6 expression is increased in fibroblastic meningioma and ovarian cancer and has been proposed as a biomarker for the latter (9, 10).
  1. Buchner, G. et al. (2000) Genomics 65:16.
  2. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
  3. Yeung, G. et al. (1999) Genomics 62:304.
  4. Chim, S.M. et al. (2011) J. Biol. Chem. 286:22035.
  5. Buchner, G. et al. (2000) Mech. Dev. 98:179.
  6. Chim, S.M. et al. (2013) Cytokine Growth Factor Rev. 24:297.
  7. Xiao, Y. et al. (2013) J. Invest. Dermatol. 133:2324.
  8. Oberauer, R. et al. (2010) Mol. Cell. Biochem. 343:257.
  9. Buckanovich, R.J. et al. (2007) J. Clin. Oncol. 25:852.
  10. Wang, X. et al. (2012) PLoS ONE 7:e52707.

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Bioinformatics

Gene Symbol EGFL6
Uniprot