Recombinant Human EG-VEGF/PK1 Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human EG-VEGF/PK1 Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using EJG bovine adrenal-derived endothelial cells. The ED₅₀ for this effect is 1-4 µg/mL. Measured in a cell proliferation assay using human pancreatic cancer cells. The ED₅₀ for this effect is 1-4 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived human EG-VEGF/PK1 protein
  Ala20-Phe105, with a C-terminal 6-His tag
• Accession #: P58294
• N-terminal Sequence: Ala20
• Protein/Peptide Type: Recombinant Proteins
• Gene: PROK1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 10.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EG-VEGF/PK1 Protein

• EGVEGF
• EG-VEGF
• EGVEGFblack mamba toxin-related protein
• Endocrine-gland-derived vascular endothelial growth factor
• Mambakine
• PK1mambakine
• PRK1prokineticin-1
• PROK1
• Prokineticin 1

Background

Endocrine gland-derived vascular endothelial growth factor (EG-VEGF), also called prokineticin 1 (PK1), is a member of the prokineticin family of secreted proteins that share a common structural motif containing ten conserved cysteine residues that form five pairs of disulfide bonds (1, 2). Members of this family include the mammalian EG-VEGF/PK1 and PK2, as well as the venom protein A (VPRA) from the venom of black mamba snake and the frog Bombina variegata, Bv8 (1). Human EG-VEGF precursor is a 105 amino acid (aa) residue protein with a 19 aa signal peptide that is cleaved to yield a 86 aa mature protein (1, 2). EG-VEGF is expressed in multiple tissues including the gastrointestinal (GI) tract and steroidogenic glands (testis, ovary, placenta and adrenal glands). EG-VEGF has been shown to potently stimulate the contraction of GI smooth muscle. In addition, EG-VEGF is a tissue-specific angiogenic factor that exhibits biological activities similar to that of VEGF on select cells. It induces the proliferation, migration, and fenestration in cultured endocrine gland-derived capillary endothelial cells. EG-VEGF binds to and activates two closely related G protein-coupled receptors, EG‑VEGF/PK1-R1 and EG-VEGF/PK2-R2 (3, 4). Activation of the receptors leads to stimulation of phosphoinositide turnover and activation of p44/p42 MAP kinase signaling pathways.

1. Li, M. et al. (2001) Mol. Pharmacol. 59:692.
2. LeCouter, J. et al. (2001) Nature 412:877.
3. Lin, D. et al. (2002) J. Biol. Chem. 277:19276.
4. Masuda, Y. et al. (2002) Biochem. Biophys. Res. Commun. 293:396.

Publications for EG-VEGF/PK1 (1209-EV)(1)

Publication using 1209-EV

• X Huang, L Jia, Z Qian, Y Jia, X Chen, X Xu, X Chang, M Liu, K Wang Diversity in human placental microvascular endothelial cells and macrovascular endothelial cells Cytokine, 2018-09-27;111(0):287-294. 2018-09-27 [PMID: 30269024] (Bioassay, Human)

Bioinformatics

• Gene Symbol: PROK1
• Uniprot:
  • P58294()