Recombinant Human EDIL3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human EDIL3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 10⁴ cells/well are added to Recombinant Human EDIL3 coated plates (5 µg/mL, 100 µL/well), >45% will adhere after 60 minutes at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human EDIL3 protein
  Val17-Glu480 with a C-terminal 6x His
• Accession #: O43854
• N-terminal Sequence: Val17, Asp24
• Protein/Peptide Type: Recombinant Proteins
• Gene: EDIL3
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EDIL3 Protein, CF

• DEL1
• DEL1developmental endothelial locus-1
• Developmentally-regulated endothelial cell locus 1 protein
• EDIL3
• EGF-like repeat and discoidin I-like domain-containing protein 3
• EGF-like repeats and discoidin I-like domains 3
• Integrin-binding protein DEL1
• MGC26287

Background

EGF‑like repeat and discoidin I‑like domain‑containing protein 3 (EDIL3; also Del‑1 and integrin‑binding DEL1) is a 52 kDa extracellular matrix protein that is expressed by endothelial tissues during embryonic vascular development (1). Human EDIL3 is synthesized as a precursor with a 16 amino acid (aa) signal sequence and a 464 aa mature chain (SwissProt # O43854). The mature chain is composed of three epidermal growth factor (EGF) repeats and two discoidin‑I‑like domains (1). The second EGF repeat contains an RGD motif (1). Splicing variants produce two isoforms for human EDIL3. Isoform 2 has an A‑>G substitution at aa 66 and a deletion of
aa 67‑76 found in isoform 1. Mature human EDIL3 shares 96% aa sequence identity with mature mouse EDIL3. The RGD motif of EDIL3 binds alpha v beta 5 integrin, which, in turn, leads to increased angiogenic transcription factor HoxD3 expression (2). HoxD3activates alpha v beta 3 and uPA, resulting in the transformation of resting endothelial cells to an angiogenic state (2‑4). EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. It has been found, however,
re‑expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis (2, 5‑6). EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through up‑regulation of Bcl‑2 expression (7). Exogenous application of EDIL3 has been demonstrated to augment angiogenesis and improve blood flow and tissue function in murine models of hind‑limb ischemia (6, 8), cardiac ischemia (9) and cerebral ischemia (2). EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA‑1‑dependent leukocyte‑endothelial adhesion (10).

1. Hidai, C. et al. (1998) Genes Dev. 12:21.
2. Fan, Y. et al. (2008) Brain Res. 1219:1.
3. Penta, K. et al. (1999) J. Biol. Chem. 274:11101.
4. Rezaee, M. et al. (2002) Am. J. Physiol. Heart Circ. Physiol. 282:H1924.
5. Aoka, Y. et al. (2002) Microvasc. Res. 64:148.
6. Ho, H.K. et al. (2004) Circulation 109:1314.
7. Pollman, M.J. et al. (1999) J. Cell Physiol. 178:359.
8. Zhong, J. et al. (2003) J. Clin. Invest. 112:30.
9. Kown, M.H. et al. (2003) Ann. Thorac. Surg. 76:1246.
10. Choi, E.Y. et al. (2008) Science 322:1101.

Publications for EDIL3/DEL1 (6046-ED)(5)

Publications using 6046-ED

• Tabasum, S;Thapa, D;Giobbie-Hurder, A;Weirather, JL;Campisi, M;Schol, PJ;Li, X;Li, J;Yoon, CH;Manos, MP;Barbie, DA;Hodi, FS; EDIL3 as an angiogenic target of immune exclusion following checkpoint blockade Cancer immunology research 2023-09-20 [PMID: 37728484] (ELISA Capture, Human)
• T Maekawa, H Tamura, H Domon, T Hiyoshi, T Isono, D Yonezawa, N Hayashi, N Takahashi, K Tabeta, T Maeda, M Oda, A Ziogas, V? Alexaki, T Chavakis, Y Terao, G Hajishenga Erythromycin inhibits neutrophilic inflammation and mucosal disease by upregulating DEL-1 JCI Insight, 2020-08-06;0(0):. 2020-08-06 [PMID: 32603314] (ELISA(Detection), Human)
• WY Kim, SH Lee, DY Kim, HJ Ryu, GR Chon, YY Park, Y Fu, JW Huh, CM Lim, Y Koh, EY Choi, SB Hong Serum developmental endothelial locus-1 is associated with severity of sepsis in animals and humans Sci Rep, 2019-09-10;9(1):13005. 2019-09-10 [PMID: 31506547] (Bioassay, ELISA Capture, Human)
• Z Kun, G Xin, W Tao, Z Chenglong, W Dongsheng, T Liang, L Tielong, X Jianru Tumor derived EDIL3 modulates the expansion and osteoclastogenesis of myeloid derived suppressor cells in murine breast cancer model J Bone Oncol, 2019-04-29;16(0):100238. 2019-04-29 [PMID: 31110935] (In Vivo, Mouse)
• SH Oh, JW Kim, Y Kim, MN Lee, MS Kook, EY Choi, SY Im, JT Koh The extracellular matrix protein Edil3 stimulates osteoblast differentiation through the integrin ?5?1/ERK/Runx2 pathway PLoS ONE, 2017-11-28;12(11):e0188749. 2017-11-28 [PMID: 29182679] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: EDIL3
• Uniprot:
  • O43854()