Recombinant Human ECE-1 Protein, CF

Images

 
There are currently no images for ECE-1 (1784-ZN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human ECE-1 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human ECE-1 protein
Gln90-Trp770, with an N-terminal signal peptide, 10-His tag and Val
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Enzymes
Gene
ECE1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
115 kDa, reducing conditions
Publications
Read Publications using
1784-ZN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 0.1 M MES, 0.1 M NaCl, pH 6.0
  • Recombinant Human ECE-1 (rhECE-1) (Catalog # 1784-ZN)
  • Fluorogenic Peptide Substrate V: MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH (Catalog # ES005)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhECE-1 to 0.1 µg/mL in Assay Buffer.
  2. Dilute Substrate to 20 µM in Assay Buffer.
  3. Load into a black well plate 50 µL of 0.1 µg/mL of rhECE-1, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate.
  4. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:
  • rhECE-1: 0.005 μg
  • Substrate: 10 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ECE-1 Protein, CF

  • EC 3.4.24
  • EC 3.4.24.71
  • ECE
  • ECE1
  • ECE-1
  • endothelin converting enzyme 1
  • endothelin-converting enzyme 1

Background

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
  3. Schweizer, A. et al. (1997) Biochem. J. 328:871.
  4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Customers Who Viewed This Item Also Viewed...

AF1182
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
NLS54
Species: Hu, Mu, Ca
Applications: WB, ICC/IF, IHC, IHC-P
AF2396
Species: Hu
Applications: WB, Simple Western, IHC
MAB5579
Species: Hu
Applications: ICC
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NB600-836
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
MAB16451
Species: Hu
Applications: WB, IHC
NBP1-87942
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-62346
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP2-66907
Species: Hu, Mu, Rt, Ze
Applications: WB, IHC, IHC-P
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-87217
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IF
H00005324-M02
Species: Hu, Mu
Applications: WB, ChIP, ELISA, IHC, IHC-P

Publications for ECE-1 (1784-ZN)(7)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Bioassay, Enzyme Assay, Western Blot.


Filter By Application
Bioassay
(3)
Enzyme Assay
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(4)
N/A
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using 1784-ZN Applications Species
Z Fang, S Chen, Y Manchanda, S Bitsi, P Pickford, A David, MM Shchepinov, IR Corrêa, DJ Hodson, J Broichhage, EW Tate, F Reimann, V Salem, GA Rutter, T Tan, SR Bloom, A Tomas, B Jones Ligand-Specific Factors Influencing GLP-1 Receptor Post-Endocytic Trafficking and Degradation in Pancreatic Beta Cells Int J Mol Sci, 2020;21(21):. 2020 [PMID: 33182425] (Bioassay, Human) Bioassay Human
AY Chen, T Tully A stress-enhanced model for discovery of disease-modifying gene: Ece1-suppresses the toxicity of ?-synuclein A30P Neurobiol. Dis., 2018;114(0):153-163. 2018 [PMID: 29524599] (Bioassay, Human) Bioassay Human
JS Miners, S Love Endothelin-converting enzymes degrade ?-synuclein and are reduced in dementia with Lewy bodies J. Neurochem, 2017;0(0):. 2017 [PMID: 28171705] (Bioassay) Bioassay
Palmer JC, Baig S, Kehoe PG, Love S Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta. Am. J. Pathol., 2009;175(1):262-70. 2009 [PMID: 19541930] (Western Blot, N/A) Western Blot N/A
Roosterman D, Kempkes C, Cottrell GS, Padilla BE, Bunnett NW, Turck CW, Steinhoff M Endothelin-converting enzyme-1 degrades internalized somatostatin-14. Endocrinology, 2008;149(5):2200-7. 2008 [PMID: 18276747] (Enzyme Assay, Human) Enzyme Assay Human
Padilla BE, Cottrell GS, Roosterman D, Pikios S, Muller L, Steinhoff M, Bunnett NW Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J. Cell Biol., 2007;179(5):981-97. 2007 [PMID: 18039931] (Enzyme Assay, N/A) Enzyme Assay N/A
Miners JS, Verbeek MM, Rikkert MO, Kehoe PG, Love S Immunocapture-based fluorometric assay for the measurement of neprilysin-specific enzyme activity in brain tissue homogenates and cerebrospinal fluid. J. Neurosci. Methods, 2008;167(2):229-36. 2008 [PMID: 17904641] (Enzyme Assay, Human) Enzyme Assay Human

Reviews for ECE-1 (1784-ZN) (0)

There are no reviews for ECE-1 (1784-ZN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for ECE-1 (1784-ZN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human ECE-1 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol ECE1
Uniprot