Recombinant Human E-Selectin/CD62E Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human E-Selectin/CD62E Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human E-Selectin coated plates (2 μg/mL with 100 μL/well), >80% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived human E-Selectin/CD62E protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
SELE
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
58.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
86-90 kDa, reducing conditions
Publications
Read Publications using
ADP1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human E-Selectin/CD62E Protein, CF

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation.

E‑Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T-cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E‑Selectin ligands on leukocytes. These interactions are labile, and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

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Publications for E-Selectin/CD62E (ADP1)(8)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Binding Assay, Bioassay, ELISA (Standard).


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Binding Assay
(1)
Bioassay
(5)
ELISA (Standard)
(1)
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Human
(6)
N/A
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using ADP1 Applications Species
J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 2019;14(7):e0219480. 2019 [PMID: 31344060] (Bioassay, Human) Bioassay Human
JH Myung, MJ Eblan, JM Caster, S Park, MJ Poellmann, K Wang, JE Tepper, KA Tam, SM Miller, C Shen, RC Chen, T Zhang, B Chera, AZ Wang, S Hong Multivalent binding and biomimetic cell rolling improves the sensitivity and specificity of circulating tumor cell�capture Clin. Cancer Res., 2018;0(0):. 2018 [PMID: 29545463] (Bioassay, Human) Bioassay Human
AK Saha, P Osmulski, SF Dallo, M Gaczynska, TH Huang, AK Ramasubram Cholesterol Regulates Monocyte Rolling through CD44 Distribution Biophys. J., 2017;112(7):1481-1488. 2017 [PMID: 28402890] (Bioassay, Human) Bioassay Human
Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis. Mol Ther, 2015;23(6):1044-54. 2015 [PMID: 25815697] (Bioassay, Human) Bioassay Human
Oliveira-Ferrer L, Rossler K, Haustein V, Schroder C, Wicklein D, Maltseva D, Khaustova N, Samatov T, Tonevitsky A, Mahner S, Janicke F, Schumacher U, Milde-Langosch K c-FOS suppresses ovarian cancer progression by changing adhesion. Br J Cancer, 2014;110(3):753-63. 2014 [PMID: 24322891]
Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat. Immunol., 2010;11(4):328-34. 2010 [PMID: 20208538] (Binding Assay, Human) Binding Assay Human
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008;7(6):2406-14. 2008 [PMID: 18422355] (ELISA (Standard), N/A) ELISA (Standard) N/A
Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release. Int. J. Cancer, 2008;123(8):1741-50. 2008 [PMID: 18324679] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol SELE
Uniprot