Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human E-Selectin/CD62E protein
Human E-Selectin
(Trp22 - Pro556)
Accession # P16581
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Trp22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SELE
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
86 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
150 kDa, reducing conditions
Publications
Read Publications using
724-ES in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 4 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

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Publications for E-Selectin/CD62E (724-ES)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Bacteria.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, Enzyme Assay Substrate, Flow Cytometry, Hemofiltration Procedure.


Filter By Application
Binding Assay
(2)
Bioassay
(19)
Cell Culture
(1)
Enzyme Assay Substrate
(1)
Flow Cytometry
(8)
Hemofiltration Procedure
(1)
All Applications
Filter By Species
Human
(28)
Mouse
(1)
Bacteria
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using 724-ES Applications Species
U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma Cancers (Basel), 2019;11(11):. 2019 [PMID: 31661833] (Bioassay, Human) Bioassay Human
A Kelkar, Y Zhu, T Groth, G Stolfa, AB Stablewski, N Singhi, M Nemeth, S Neelamegha Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing Mol. Ther., 2019;0(0):. 2019 [PMID: 31601489] (Flow Cytometry, Human) Flow Cytometry Human
N Jyotsana, Z Zhang, LE Himmel, F Yu, MR King Minimal dosing of leukocyte targeting TRAIL decreases triple-negative breast cancer metastasis following tumor resection Sci Adv, 2019;5(7):eaaw4197. 2019 [PMID: 31355333] (Cell Culture, Human) Cell Culture Human
JF Osborn, SJ Hobbs, JL Mooster, TN Khan, AM Kilgore, JC Harbour, JC Nolz Central memory CD8+ T cells become CD69+ tissue-residents during viral skin infection independent of CD62L-mediated lymph node surveillance PLoS Pathog., 2019;15(3):e1007633. 2019 [PMID: 30875408] (Binding Assay, Human) Binding Assay Human
HA Cuello, VI Segatori, M Albertó, CA Gulino, R Aschero, S Camarero, LG Mutti, K Madauss, DF Alonso, F Lubienieck, MR Gabri Aberrant O-glycosylation modulates aggressiveness in neuroblastoma Oncotarget, 2018;9(75):34176-34188. 2018 [PMID: 30344930] (Bioassay, Human) Bioassay Human
K Kitamura, K Sato, M Sawabe, M Yoshida, N Hagiwara P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome Circ. J., 2018;0(0):. 2018 [PMID: 29962384] (Bioassay, Human) Bioassay Human
NM Cordina, N Sayyadi, LM Parker, A Everest-Da, LJ Brown, NH Packer Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates Sci Rep, 2018;8(1):4521. 2018 [PMID: 29540838] (Bioassay) Bioassay
CH Lee, HH Zhang, SP Singh, L Koo, J Kabat, H Tsang, TP Singh, JM Farber C/EBP? drives interactions between human MAIT cells and endothelial cells that are important for extravasation Elife, 2018;7(0):. 2018 [PMID: 29469805] (Bioassay, Human) Bioassay Human
FW Lam, Q Da, B Guillory, MA Cruz Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium J. Immunol., 2018;0(0):. 2018 [PMID: 29335256] (Bioassay, Human) Bioassay Human
Lara Toffali SOS1, ARHGEF1, and DOCK2 rho-GEFs Mediate JAK-Dependent LFA-1 Activation by Chemokines J. Immunol, 2016;0(0):. 2016 [PMID: 27986909] (Bioassay, Human) Bioassay Human
Show All 32 Publications.

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Bioinformatics

Gene Symbol SELE
Uniprot