Recombinant Human Desmocollin-1 Protein, CF

Images

 
There are currently no images for .

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human Desmocollin-1 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells. When 3 x 104 cells/well are added to Recombinant Human Desmocollin-1 coated plates (10 µg/mL, 100 µL/well), >45% will adhere after 90 minutes at 37 °C.
Source
Mouse myeloma cell line, NS0-derived
Arg135-Asn686, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg135
Protein/Peptide Type
Recombinant Proteins
Gene
DSC1
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-85 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from 0.2 μm filtered solution in PBS.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Desmocollin-1 Protein, CF

  • cadherin family member 1
  • CDHF1
  • desmocollin 1
  • Desmocollin1
  • Desmocollin-1
  • desmosomal glycoprotein 2/3
  • DG2/DG3
  • DG2/DG3
  • DSC1

Background

Desmocollin-1 (DSC1) is a 100 kDa-110 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules (1-3). Human DSC1 is synthesized with a 105 amino acid (aa) propeptide. The mature protein consists of a 557 aa extracellular domain (ECD) that contains five cadherin-like domains, a 23 aa transmembrane segment, and a 180 aa cytoplasmic domain (4, 5). Within the ECD, human DSC1 shares 79% and 82% aa sequence identity with bovine and mouse DSC1, respectively, and 53% with human DSC2 and DSC3. An alternately spliced isoform has a substituted and truncated cytoplasmic domain (5). DSC1 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is predominantly expressed in the outer layers of stratified epithelia of the skin, tongue, and hair follicle root sheath (6, 7). It is required for both promoting epidermal differentiation and maintaining epidermal strength (8). DSC2 and DSC3, by contrast, are preferentially localized in the basal and suprabasal layers of the epidermis (1). DSC1 is not expressed in normal colon epithelium, but it is induced during colon carcinogenesis (9). Loss of DSC1 function in the skin disorders IgA pemphigus and Netherton Syndrome results from autoimmune targeting or enhanced KLK-7 mediated proteolysis, respectively (10-12).

Customers Who Viewed This Item Also Viewed...

MAB944
Species: Hu
Applications: WB, IHC
MAB7265
Species: Mu
Applications: WB, IHC
AF7490
Species: Mu
Applications: IHC
NBP1-78984
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-49879
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
AF5725
Species: Hu
Applications: WB, IHC
AF947
Species: Hu
Applications: WB, Simple Western
AF2624
Species: Hu, Mu
Applications: WB, IHC, IP
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-32962
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP2-34270
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NB100-56546
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-90042
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2008
Species: Hu
Applications: WB, IHC, IP
NB100-2727
Species: Hu, Po, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP1-85731
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
46780002
Species: Hu
Applications: ELISA
NBP1-90509
Species: Hu
Applications: ICC/IF, IHC, IHC-P
4955-DC
Species: Hu
Applications: BA

Publications for Desmocollin-1 (0)

There are no publications for Desmocollin-1.
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Desmocollin-1 (0)

There are no reviews for Desmocollin-1. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Desmocollin-1 (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Desmocollin-1 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol DSC1
Entrez
Uniprot