Recombinant Human CXCL2/GRO beta (aa 39-107) Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CXCL2/GRO beta (aa 39-107) Protein, CF Summary

• Details of Functionality: Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED₅₀ for this effect is 0.1-0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED₅₀ for this effect is 1-5 ng/mL.
• Source: E. coli-derived human CXCL2/GRO beta/MIP-2/CINC-3 protein
  Thr39-Asn107
• Accession #: NP_002080
• N-terminal Sequence: Thr39
• Protein/Peptide Type: Recombinant Proteins
• Gene: CXCL2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 7.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL2/GRO beta (aa 39-107) Protein, CF

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• CINC3
• CINC-3
• C-X-C Motif Chemokine 2
• CXCL2
• GRO beta
• GRO2 oncogene
• GRO2
• GROB
• Gro-beta
• Growth-regulated protein beta
• Macrophage inflammatory protein 2-alpha
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MGSA-beta
• MIP2
• MIP-2
• MIP2A
• MIP-2a
• MIP2-alpha
• SCYB2

Background

Human GRO alpha , GRO beta (MIP-2 alpha ), and GRO gamma (MIP-2 beta ) are products of three distinct, non-allelic human genes. GRO beta and GRO gamma share 90% and 86% amino acid (aa) sequence homology, respectively, with GRO alpha . All three human GROs are members of the alpha (C-X-C) subfamily of chemokines and are thought to be the homologs of the murine KC, and MIP-2.

The three GRO cDNAs encode 107 aa precursor proteins from which the N-terminal 34 aa residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the aa sequences. GRO expression is inducible by serum, PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.

Similarly to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to CXCR2. The 69 aa variant of human GRO beta is approximately 3 times more active than the 73 aa variant (R&D Systems, Catalog # 276-GB) in inducing myeloperoxidase release from cytochalasin B treated neurotrophils.

1. Chem. Immunol. (1999) 72:1.

Publications for CXCL2/GRO beta/MIP-2/CINC-3 (176-GB/CF)(1)

Publication using 176-GB/CF

• Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2006-12-29;282(16):11658-66. 2006-12-29 [PMID: 17197447] (Binding Assay, Mouse)

Bioinformatics

• Gene Symbol: CXCL2
• Uniprot:
  • NP_002080()