Recombinant Human CTGF/CCN2 Protein, CF

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Immobilized Recombinant Human CTGF (Catalog # 9190-CC)supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50for this effect is1.5-7.5 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human CTGF/CCN2 Protein, CF Summary

Details of Functionality
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. The ED50 for this effect is1.5-7.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CTGF/CCN2 protein
Gln27-Ala349
Accession #
N-terminal Sequence
No results obtained. Gln27 inferred from enzymatic pyroglutamate treatment revealing Asn28
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35-45 kDa, reducing conditions
Publications
Read Publications using
9190-CC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CTGF/CCN2 Protein, CF

  • CCN2
  • CCN2IGFBP-8
  • connective tissue growth factor
  • CTGF
  • CTGRP
  • Fisp12
  • HCS24
  • Hypertrophic chondrocyte-specific protein 24
  • IBP-8
  • IGF-binding protein 8
  • IGFBP-8
  • IGFBP8CCN family member 2
  • Insulin-like growth factor-binding protein 8
  • MGC102839
  • NOV2

Background

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Human CTGF shares 94% amino acid (aa) sequence identity with mouse and rat CTGF. Alternative splicing generates an additional isoform with a 27 aa deletion within the TSP-1 domain. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins  alpha M, alpha V beta 3, alpha 6 beta 1, alpha IIb beta 3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).
  1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
  2. Bradham, D.M. et al. (1991) J. Cell Biol. 114:1285.
  3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.
  4. Schober, J.M. et al. (2002) Blood 99:4457.
  5. Heng, E.C.K. et al. (2006) J. Cell Biochem. 98:409.
  6. Jedsadayanmata, A. et al. (1999) J. Biol. Chem. 274:24321.
  7. Gao, R. et al. (2003) Hepatol. Res. 27:214.
  8. Mercurio, S. et al. (2004) Development 131:2137.
  9. Wahab, N.A. et al. (2005) J. Am. Soc. Nephrol. 16:340.
  10. Abreu, J.G. et al. (2002) Nat. Cell Biol. 4:599.
  11. Hashimoto, G. et al. (2002) J. Biol. Chem. 277:36288.
  12. Khodosevich, K. et al. (2013) Neuron 79:1136.
  13. Shi-Wen, X. et al. (2006) J. Biol. Chem. 281:10715.
  14. Lee, C.H. et al. (2010) J. Clin. Invest. 120:3340.
  15. Canalis, E. et al. (2010) Endocrinology 151:3490.
  16. Nakanishi, T. et al. (2000) Endocrinology 141:264.
  17. Ivkovic, S. et al. (2003) Development 130:2779.
  18. Hall-Glenn, F. et al. (2012) PLoS One 7:e30562.
  19. Shimo, T. et al. (1999) J. Biochem. 126:137.

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Publications for CTGF/CCN2 (9190-CC)(2)

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FAQs for CTGF/CCN2 (9190-CC). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
    • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

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