Recombinant Human Complement Factor H-related 5/CFHR5, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Complement Factor H-related 5/CFHR5, CF Summary

• Details of Functionality: Measured by its ability to inhibit the binding of biotinylated Recombinant Human Complement Factor H-related 5/CFHR5 to immobilized Recombinant Human C‑Reactive Protein/CRP (Catalog # 1707-CR). McRae, J.L. et al. (2005) J. Immunol. 174:6250.
• Source: Mouse myeloma cell line, NS0-derived human Complement Factor H-related 5/CFHR5 protein
  Glu19-Glu569, with a C-terminal 10-His tag
• Accession #: NP_110414
• N-terminal Sequence: Glu19
• Protein/Peptide Type: Recombinant Enzymes
• Gene: CFHR5
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 73 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Complement Factor H-related 5/CFHR5, CF

• CFHL5
• CFHL5factor H-related protein 5
• CFHR5
• Complement Factor Hrelated 5
• Complement Factor H-related 5
• FHR5
• FHR-5complement factor H-related protein 5
• FHR5MGC133240
• FLJ10549

Background

The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). FHR-5 has been identified initially as a universal component of complement deposits (1), and detected in glomerular immune deposits (4). The pattern of deposits is similar to other complement components, suggesting that FHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. Its biological function is not understood fully. FHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association (5). Weak factor I-dependent cofactor activity for C3b cleavage has also been observed (5).

1. McRae, J. L. et al. (2001) J. Biol. Chem. 276:6747.
2. Zipfel, P. F. et al. (2002) Biochem. Soc. Trans. 30:971.
3. McRae, J. L. et al. (2002) Genetica. 114:157.
4. Murphy, B. et al. (2002) Am. J. Kidney. Dis. 39:24.
5. McRae, J. L. et al. (2005) J. Immunol. 174:6250.

Publications for Complement Factor H-related 5/CFHR5 (3845-F5)(7)

Publications using 3845-F5

• Jeon, C;Kim, D;Kim, KM;Lee, SH;Lee, JH;Kim, SH;Kim, JS;Kang, YM;Jo, S;Kim, TH;Son, CN; Complement factor H-related protein 5 alleviates joint inflammation and osteoclast differentiation by disrupting RANK-JNK signaling in collagen antibody-induced arthritis mouse model Cytokine 2024-10-25 [PMID: 39461285] (In vivo assay, Mouse)
• Zouache, MA;Richards, BT;Pappas, CM;Anstadt, RA;Liu, J;Corsetti, T;Matthews, S;Seager, NA;Schmitz-Valckenberg, S;Fleckenstein, M;Hubbard, WC;Thomas, J;Hageman, JL;Williams, BL;Hageman, GS; Levels of complement factor H-related 4 protein do not influence susceptibility to age-related macular degeneration or its course of progression Nature communications 2024-01-10 [PMID: 38200010] (ELISA, Human)
• Iglesias, MJ;Sanchez-Rivera, L;Ibrahim-Kosta, M;Naudin, C;Munsch, G;Goumidi, L;Farm, M;Smith, PM;Thibord, F;Kral-Pointner, JB;Hong, MG;Suchon, P;Germain, M;Schottmaier, W;Dusart, P;Boland, A;Kotol, D;Edfors, F;Koprulu, M;Pietzner, M;Langenberg, C;Damrauer, SM;Johnson, AD;Klarin, DM;Smith, NL;Smadja, DM;Holmström, M;Magnusson, M;Silveira, A;Uhlén, M;Renné, T;Martinez-Perez, A;Emmerich, J;Deleuze, JF;Antovic, J;Soria Fernandez, JM;Assinger, A;Schwenk, JM;Souto Andres, JC;Morange, PE;Butler, LM;Trégouët, DA;Odeberg, J; Elevated plasma complement factor H related 5 protein is associated with venous thromboembolism Nature communications 2023-06-07 [PMID: 37286573] (ELISA, Western Blot, Human)
• WY Guo, LJ Sun, HR Dong, GQ Wang, XY Xu, ZR Zhao, H Cheng Glomerular Complement Factor H-Related Protein 5 is Associated with Histologic Injury in Immunoglobulin A Nephropathy Kidney international reports, 2020-11-21;6(2):404-413. 2020-11-21 [PMID: 33615066] (ELISA Control, Human)
• NR Medjeral-T, H Moffitt, HJ Lomax-Brow, N Constantin, T Cairns, HT Cook, MC Pickering Glomerular Complement Factor H-Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment Kidney Int Rep, 2019-06-19;4(10):1387-1400. 2019-06-19 [PMID: 31701048] (IHC-P, Human)
• ÁI Csincsi, Z Szabó, Z Bánlaki, B Uzonyi, M Cserhalmi, É Kárpáti, A Tortajada, JJE Caesar, Z Prohászka, TS Jokiranta, SM Lea, S Rodríguez, M Józsi FHR-1 Binds to C-Reactive Protein and Enhances Rather than Inhibits Complement Activation J. Immunol., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533443] (Bioassay)
• Csincsi A, Kopp A, Zoldi M, Banlaki Z, Uzonyi B, Hebecker M, Caesar J, Pickering M, Daigo K, Hamakubo T, Lea S, Goicoechea de Jorge E, Jozsi M Factor H-related protein 5 interacts with pentraxin 3 and the extracellular matrix and modulates complement activation. J Immunol, 2015-04-08;194(10):4963-73. 2015-04-08 [PMID: 25855355] (Binding Assay, Human)

Bioinformatics

• Gene Symbol: CFHR5
• Uniprot:
  • NP_110414()