Recombinant Human Chordin-Like 1 Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human Chordin-Like 1 Protein Summary

• Details of Functionality: Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.7-3.5 µg/mL in the presence of 50 ng/mL Recombinant Human BMP-4 Recombinant Human BMP‑4 (Catalog # 314-BP) and 1 µg/mL of Recombinant Human TSG (Catalog # 6684-TG).
• Source: Mouse myeloma cell line, NS0-derived human Chordin-like 1/CHRDL1 protein
  Glu22-Cys450, with a C-terminal 10-His tag
• Accession #: Q9BU40.2
• N-terminal Sequence: Glu22
• Protein/Peptide Type: Recombinant Proteins
• Gene: CHRDL1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Chordin-Like 1 Protein

• CHL
• Chordin-like 1
• chordin-like protein 1
• CHRDL1
• CRDL1
• dA141H5.1
• Neuralin 1
• neuralin-1
• neurogenesin-1
• NRLN1
• NRLN1neuralin 1
• Ventroptin alpha
• ventroptin
• VOPT

Background

Chordin-like 1 (CHRDL1) also known as Chordin-like, ventroptin, and neuralin, is a secreted glycoprotein that has significant homology to chordin and acts as a bone morphogenetic protein (BMP) antagonist (1-3). Human Chordin-Like 1 cDNA encodes a 450 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Chordin-Like 1 contains three internal cysteine-rich procollagen repeats (CRs) that are conserved in the spacing of their ten cysteines. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding (1). The CR1 and CR3 of Chordin-Like 1 are most similar to CR3 of chordin (1). Chordin-Like 1 and chordin exhibit similar BMP inhibitory activity in vivo and directly interact with BMP-4 in vitro (1, 2). However, Chordin-Like 1 differs in its spatial and temporal expression from chordin. Mouse Chordin-Like 1 is detected in mesenchyme-derived cells such as the dermatome and limb bud, as well as chondrocytes of the developing skeleton later in development. In addition, Chordin-Like 1 is expressed embryonically in the gastrointestinal tract, kidney tubules, neural tube, CNS, cerebellum, derivatives of the neural crest cells, developing hair follicles, and the olfactory bulb where it is expressed in a gradient in the retina
(1-3). Chordin-Like 1 is also expressed in adult marrow stromal cells. Human CHRDL1 and the long form of mouse CHRDL1 share 93% aa sequence identity.

Multiple forms of human Chordin-Like 1 containing small deletions or insertions (including an E insertion between P94 and D95, a GKKAK deletion (aa residues
318-322), and/or another E insertion between K322 and E323) have been described. Similar isoforms have also been reported for mouse Chordin-Like 1. In addition, a short form of mouse Chordin-Like 1 with a shortened variant C-terminus but with all 3 CR domains intact also exists (1). These multiple forms were attributed to alternative splicing and transcriptional termination sites within the coding region of Chordin-Like 1.

1. Nakayama, N. et al. (2001) Dev. Biol. 232:372.
2. Sakuta, H. et al. (2001) Science 293:111.
3. Coffinier, C. et al. (2001) Mech. Dev. 100:119.

Publications for Chordin-like 1/CHRDL1 (1808-NR)(7)

Publications using 1808-NR

• O'Sullivan, JDB;Blacker, TS;Scott, C;Chang, W;Ahmed, M;Yianni, V;Mann, ZF; Gradients of glucose metabolism regulate morphogen signalling required for specifying tonotopic organisation in the chicken cochlea eLife 2023-08-04 [PMID: 37539863] (Bioassay, Chicken)
• Mann Z, Thiede B, Chang W, Shin J, May-Simera H, Lovett M, Corwin J, Kelley M A gradient of Bmp7 specifies the tonotopic axis in the developing inner ear. Nat Commun, 2014-05-20;5(0):3839. 2014-05-20 [PMID: 24845721] (Bioassay, Chicken)
• Olsen O, Wader K, Misund K, Vatsveen T, Ro T, Mylin A, Turesson I, Stordal B, Moen S, Standal T, Waage A, Sundan A, Holien T Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin. Blood Cancer J, 2014-03-21;4(0):e196. 2014-03-21 [PMID: 24658374] (Bioassay, Human)
• Zhang Y, Liu J, Tian X, Wong W, Chen Y, Wang L, Luo J, Cheang W, Lau C, Kwan K, Wang N, Yao X, Huang Y Inhibition of bone morphogenic protein 4 restores endothelial function in db/db diabetic mice. Arterioscler Thromb Vasc Biol, 2013-11-07;34(1):152-9. 2013-11-07 [PMID: 24202302] (Bioassay, Mouse)
• Talavera-Adame , Dodanim, Gupta , Ankur, Kurtovic , Silvia, Chaiboonma , Kira L, Arumugaswami , Vaithili, Dafoe , Donald C Bone morphogenetic protein-2/-4 upregulation promoted by endothelial cells in coculture enhances mouse embryoid body differentiation. Stem Cells Dev, 2013-09-24;22(24):3252-60. 2013-09-24 [PMID: 23924071] (Bioassay, Mouse)
• Alborzinia H, Schmidt-Glenewinkel H, Ilkavets I, Breitkopf-Heinlein K, Cheng X, Hortschansky P, Dooley S, Wolfl S Quantitative kinetics analysis of BMP2 uptake into cells and its modulation by BMP antagonists. J Cell Sci, 2012-10-17;126(0):117-27. 2012-10-17 [PMID: 23077176] (Bioassay, Human)
• Kane R, Godson C, O'Brien C Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis. Mol. Vis., 2008-06-20;14(0):1138-48. 2008-06-20 [PMID: 18587495] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CHRDL1
• Uniprot:
  • Q9BU40.2()