Recombinant Human Chondroadherin His-tag Protein, CF

Recombinant Human Chondroadherin (Catalog # 8218-CH) inducesadhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4-2.4 μg/mL.

2 μg/lane of Recombinant Human Chondroadherin was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 33-38 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Chondroadherin His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.4-2.4 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Chondroadherin protein
  Ala22-Thr350, with a C-terminal 6-His tag
• Accession #: O15335
• N-terminal Sequence: Ala22 & Cys23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 33-38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Chondroadherin His-tag Protein, CF

• CHAD
• chondroadherin proteoglycan
• Chondroadherin
• SLRR4A
• SLRR4ACartilage leucine-rich protein

Background

Chondroadherin (CHAD), also known as SLRR4A, is a 38-kDa secreted protein belonging to the small leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function of the ECM (1). CHAD is highly expressed in cartilaginous tissues, with lower expression levels found in bone, tendon, and eye (2-4). Mature human CHAD consists of a 337 amino acid (aa) protein that contains eleven leucine-rich repeats (LRRs) including nine tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich regions (5). CHAD interacts with collagen II and mediates signaling between chondrocytes and the ECM by binding to the alpha 2 beta 1 integrin, heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In addition, CHAD interacts with both N- and C-terminal globular domains of type VI collagen (10). Human CHAD shares 95% and 94% aa sequence identity with mouse and rat CHAD, respectively.

1. Neame, P.J. et al. (1994) J. Biol. Chem. 269:21547.
2. Larson, T. et al. (1991) J. Biol. Chem. 266:20428.
3. Mizuno, M. et al. (1996) Calcif. Tissue Int. 59:163.
4. Johnson, J.M. et al. (2006) Mol. Vis. 12:1057.
5. Månsson, B. et al. (2001) J. Biol. Chem. 276:32883.
6. Paracuellos, P. et al. (2017) Matrix Biol. 63:106.
7. Batista, M. et al. (2014) Matrix Biol. 38:84.
8. Camper, L. et al. (1997) J. Cell Biol. 138:1159.
9. Haglund, L. et al. (2013) J. Biol. Chem. 288:995.
10. Wiberg, C. et al. (2002) J. Biol. Chem. 277:49120.

Bioinformatics

• Uniprot:
  • O15335()