Recombinant Human CHIP/STUB1 Protein, CF

Product Discontinued

Recombinant Human CHIP/STUB1 Protein, CF Summary

• Details of Functionality: Recombinant Human CHIP/Stub1 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human CHIP/Stub1 concentration of 0.1-5 μM.
• Source: E. coli-derived human CHIP/STUB1 protein
  Contains a C-terminal 6-His tag
• Accession #: Q9UNE7
• Protein/Peptide Type: Recombinant Enzymes
• Gene: STUB1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% Glycerol (v/v), 5 mM DTT
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CHIP/STUB1 Protein, CF

• Antigen NY-CO-7
• Carboxy terminus of Hsp70-interacting protein
• CHIP
• CHIPSTIP1 homology and U box-containing protein 1
• CLL-associated antigen KW-8
• E3 ubiquitin-protein ligase CHIP
• EC 6.3.2.-
• heat shock protein A binding protein 2 (c-terminal)
• HSPABP2
• NY-CO-7
• SDCCAG7
• serologically defined colon cancer antigen 7
• STIP1 homology and U-box containing protein 1
• STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
• STUB1
• UBOX1

Background

STIP1 Homology and U-box Containing Protein 1 (STUB1), also known as Carboxyl Terminus of Hsp70-interacting Protein (CHIP), is a cytoplasmic protein that functions as a U-box Ubiquitin ligase (E3) (1). It is highly expressed in striated muscle and brain and has been observed at lower levels in other organs including the pancreas, lung, liver, and kidney (2,3). STUB1/CHIP is 303 amino acids (aa) in length with a predicted molecular weight of 34.8 kDa (2). Human STUB1/CHIP shares 97% aa sequence identity with the mouse ortholog but only 67% sequence identity with the rat ortholog (2). It consists of three 34 aa N-terminal tetratricopeptide repeat (TRP) domains (aa 26-127) that are responsible for protein-protein interactions and a C-terminal U-box domain (aa 226-300) that participates in ubiquitination (1,4,5). A central domain containing charged residues lies between the TRP and U-box domains and is thought to be necessary for TRP-dependent interactions (3). STUB1/CHIP participates in intracellular protein folding/refolding and degradation. It complexes with several molecular chaperone proteins, including HSP70/HSPA1A, HSC70, and HSP90, and modulates their activity (2,3,5,6). It also facilitates the ubiquitination of chaperone substrates, including nascent CFTR, phosphorylated Tau, p53, PTEN, Synuclein-alpha , and beta -APP, promoting their degradation (5,7-11). This recombinant human protein contains a C-terminal His₆ tag.

1. Jiang, J. et al. (2001) J. Biol. Chem. 276:42938.
2. Ballinger, C.A. et al. (1999) Mol. Cell. Biol. 19:4535.
3. McDonough, H. & C. Patterson (2003) Cell Stress Chaperones 8:303.
4. Schulman, B.A. & Z.J. Chen (2005) Mol. Cell 20:653.
5. Kumar, P. et al. (2012) J. Neurochem. 120:350.
6. Connell, P. et al. (2001) Nat. Cell Biol. 3:93.
7. Meacham, G.C. et al. (2001) Nat. Cell Biol. 3:100.
8. Shin, Y. et al. (2005) J. Biol. Chem. 280:23727.
9. Esser, C. et al. (2005) J. Biol. Chem. 280:27443.
10. Kumar, P. et al. (2007) Hum. Mol. Genet. 16:848.
11. Ahmed, S.F. et al. (2012) J. Biol. Chem. 287:15996.

Publications for CHIP/STUB1 (E3-220)(2)

Publications using E3-220

• MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage Cell Death & Disease, 2020-09-10;11(9):740. 2020-09-10 [PMID: 32913203] (Ubiquitylation, Human)
• JH Seo, E Agarwal, KG Bryant, MC Caino, ET Kim, AV Kossenkov, HY Tang, LR Languino, DI Gabrilovic, AR Cohen, DW Speicher, DC Altieri Syntaphilin Ubiquitination Regulates Mitochondrial Dynamics And Tumor Cell Movements Cancer Res., 2018-06-13;0(0):. 2018-06-13 [PMID: 29898993] (Bioassay)

Bioinformatics

• Gene Symbol: STUB1
• Uniprot:
  • Q9UNE7(Human)