Recombinant Human CEACAM-7 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CEACAM-7 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM-7 is immobilized at 0.1 µg/mL (100 µL/well), the concentration of Recombinant Human
  CEACAM-1/CD66a (Catalog # 2244-CM) that produces 50% of the optimal binding response is approximately 1.6-8 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CEACAM-7 protein
  Thr36-Asn233, with a C-terminal 6-His tag
• Accession #: Q14002
• N-terminal Sequence: Thr36
• Protein/Peptide Type: Recombinant Proteins
• Gene: CEACAM7
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 42-52 kDa, reducing conditions

Packaging, Storage & Formulations

• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CEACAM-7 Protein, CF

• Carcinoembryonic antigen CGM 2
• Carcinoembryonic antigen CGM2
• Carcinoembryonic antigen gene family member 2
• Carcinoembryonic antigen related cell adhesion molecule 7
• CEA
• CEACAM 7
• CEACAM7
• CEACAM-7
• CGM 2
• CGM2

Background

Carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM-7), also known as CGM2, is an approximately 40 kDa GPI-anchored glycoprotein in the CEACAM family of adhesion molecules (1). Mature human CEACAM-7 consists of two Ig-like domains followed by the GPI anchor (2). Alternative splicing generates a short isoform that lacks the second Ig-like domain. CEACAM-7 is preferentially expressed on the luminal surface of epithelial cells near the mouth of colonic crypts and on pancreatic ductal epithelial cells (3, 4). It is down-regulated during colorectal adenoma progression (2-6) but can be up-regulated during the development of gastric carcinoma (7). R&D Systems in-house testing indicates that CEACAM-7 binds to CEACAM-1, consistent with the heterophilic interaction of CEACAM-1 with other CEACAM family members (1, 8, 9).

1. Tchoupa, A.K. et al. (2014) Cell Commun. Signal. 12:27.
2. Thompson, J. et al. (1994) J. Biol. Chem. 269:32924.
3. Thompson, J. et al. (1997) Cancer Res. 57:1776.
4. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
5. Nollau, P. et al. (1997) Cancer Res. 57:2354.
6. Nollau, P. et al. (1997) Am. J. Pathol. 151:521.
7. Zhou, J. et al. (2011) World J. Surgical Oncol. 9:172.
8. Markel, G. et al. (2004) J. Immunol. 173:3732.
9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.

Bioinformatics

• Gene Symbol: CEACAM7
• Uniprot:
  • Q14002(Human)