Recombinant Human CEACAM-3/CD66d His-tag Protein, CF

When Recombinant Human CEACAM‑3/CD66d (Catalog # 9868‑CM) is coated at 2 μg/mL, Recombinant Human CEACAM‑7 (Catalog # 9010‑CM) binds with an ED50 of 0.3‑3.6 μg/mL.

2 μg/lane of Recombinant Human CEACAM‑3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 19‑25 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CEACAM-3/CD66d His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM‑3/CD66d is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human CEACAM-7 (Catalog # 9010-CM) that produces 50% of the optimal binding response is 0.6-3.6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human CEACAM-3/CD66d protein
  Lys35-Gly155, with a C-terminal 6-His tag
• Accession #: P40198-1
• N-terminal Sequence: Lys35
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19-25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CEACAM-3/CD66d His-tag Protein, CF

• CD66d
• CEA
• CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3
• CEACAM-3
• CGM1
• W264
• W282

Background

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3 (CEACAM‑3), or CD66d, is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)‑linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM‑3 is approximately 35 kDa, consisting of an extracellular domain (ECD) containing one IgV‑like domain, a single transmembrane domain and a cytoplasmic tail. The cytoplasmic tail of CEACAM‑3 contains an immunoreceptor tyrosine-based activation motif (ITAM), which recruits kinases to propagate pro-inflammatory signaling cascades (3). CEACAM‑3 appears to be primate specific, with no non-primate orthologs currently identified (4). Originally discovered as a biomarker for colorectal cancer (5), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (6-8). Unlike other CEA family members, CEACAM‑3 has not been shown to form cell–cell adhesion interactions with other CEACAM family members (9). CEACAM‑3 has been found to be specifically expressed on human neutrophils and other granulocytes and appears to be ann adaptation of the innate immune system to handle specific host pathogens (9). The granulocyte-specific CEACAM epitope is present on at least four CEA family members, CEACAM‑1 -3, -6 and -8, which upon engagement on the neutrophil surface triggers a transient activation signal that requires extracellular calcium and regulates the adhesive activity of the beta2 integrin, CD11/CD18 (10, 11). CEACAM‑3 is critical for opsonin‑independent phagocytosis and bacterial clearance (3). CEACAM‑3 binds to the colony opacity-associated (Opa) outer membrane proteins of bacteria, such as Neisseria gonorrhoeae, and triggers uptake of the pathogen and subsequent elimination (3, 9).

1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
2. Zebhauser, R. et al. (2005) Genomics 86:566.
3. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.
4. Pavlopoulou A. and Scorilas A. 2014 Genome Biol Evol. 6(6):1314.
5. Gold P and Freedman SO, (1965) J Exp Med 122:467.
6. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
7. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol 283.
8. Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
9. Pils S. et al. (2008) Int. J. of Med. Micro. 298, 7–8:553.
10. Nagel G. et al. (1993) Eur J Biochem 214:27.
11. Skubitz KM et al. (1996) J Leukoc Biol 60:106.

Bioinformatics

• Uniprot:
  • P40198-1()