Recombinant Human CD55/DAF Protein

Images

 
There are currently no images for CD55/DAF (2009-CD).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human CD55/DAF Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA.

When Recombinant Human (rh) CD55/DAF is coated at 1 μg/mL (100 μL/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 μg/mL.

Source
Mouse myeloma cell line, NS0-derived human CD55/DAF protein
Human CD55
(Asp35 - Ser353)
Accession # P08174.4
DI 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp35
Protein/Peptide Type
Recombinant Proteins
Gene
CD55
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
61-75 kDa, reducing conditions
Publications
Read Publications using
2009-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD55/DAF Protein

  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • CD55
  • CR
  • CRdecay accelerating factor for complement (CD55, Cromer blood group system)
  • CROMDAFcomplement decay-accelerating factor
  • DAF
  • decay accelerating factor for complement
  • TC

Background

CD55, also known as DAF or decay-accelerating factor, is a 70 - 75 kDa member of the RCA family of proteins. Human RCA (regulators of complement/C’ activation) proteins are products of chromosome 1 genes that are ubiquitously expressed on cells exposed to plasma complement proteins (1 - 4). A hallmark of RCA proteins is the presence of four to 30 SCRs (short consensus repeats; also called CCPs for C’ control protein modules) in their plasma-exposed regions. SCRs are characterized by a 60 - 65 amino acid (aa) module that contains a highly conserved Trp residue and two internal disulfide bonds that create a beta -barrel structure (1). Human CD55 is synthesized as a 381 aa precursor that contains a 34 aa signal sequence, a 319 aa mature region and a 28 aa C‑terminal prosegment (5, 6). The mature region contains four SCR modules and a C‑terminal O‑glycosylated extension (7). Following cleavage of the prosegment, a serine is exposed that serves as an anchor for a GPI-linkage (8). Multiple polymorphisms are found in the molecule. Alternate splicing also exists. One form that may not be translated shows an intron insertion in the prosegment, resulting in a 79 aa substitution for the standard C‑terminal 20 aas of the prosegment (6). Another form generates a truncated 199 aa precursor that cannot be membrane-bound and may not be secreted (9). Mature CD55 is 53% and 84% aa identical to mouse and monkey CD55, respectively. CD55 is known to bind CD97 via the first SCR (4). It also binds physiologically-generated C3 convertases with its second and third SCRs (7, 10). Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on nonforeign cells (1, 2). Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection (4).

  1. Herbert, A. et al. (2002) Biochem. Soc. Trans. 30:990. 
  2. Miwa, T. and W-C. Song (2001) Int. Immunopharmacol. 1:445. 
  3. Hourcade, D. et al. (2000) Immunopharmacology 49:103. 
  4. Lea, S. (2002) Biochem. Soc. Trans. 30:1014. 
  5. Medof, M.E. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2007. 
  6. Caras, I.W. et al. (1987) Nature 325:545.
  7. Lukacik, P. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1279.
  8. Moran, P. et al. (1991) J. Biol. Chem. 266:1250.
  9. Lublin, D.M. et al. (1994) Blood 84:1276.
  10. Williams, P. et al. (2003) J. Biol. Chem. 278:10691.

Customers Who Viewed This Item Also Viewed...

H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF
AF1107
Species: Mu
Applications: WB, IHC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NB500-330
Species: Hu
Applications: Flow, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready
MAB2005
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB200-540
Species: Mu
Applications: WB, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-47974
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-20383
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90213
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37477
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-52979
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for CD55/DAF (2009-CD)(4)

We have publications tested in 3 confirmed species: Mouse, Rat, Bacteria.

We have publications tested in 2 applications: In Vivo, Substrate for Enzyme Digestion.


Filter By Application
In Vivo
(3)
Substrate for Enzyme Digestion
(1)
All Applications
Filter By Species
Mouse
(2)
Rat
(1)
Bacteria
(1)
All Species

Reviews for CD55/DAF (2009-CD) (0)

There are no reviews for CD55/DAF (2009-CD). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CD55/DAF (2009-CD) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for CD55/DAF (2009-CD)

Discover related pathways, diseases and genes to CD55/DAF (2009-CD). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD55/DAF (2009-CD)

Discover more about diseases related to CD55/DAF (2009-CD).
 

Pathways for CD55/DAF (2009-CD)

View related products by pathway.

PTMs for CD55/DAF (2009-CD)

Learn more about PTMs related to CD55/DAF (2009-CD).

Blogs on CD55/DAF

There are no specific blogs for CD55/DAF, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CD55/DAF Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol CD55
Uniprot