Recombinant Human CD43 Protein, CF

Recombinant Human CD43 binds to Recombinant Human Siglec-1/CD169 (Catalog # 5197-SL) binds with an ED50 of 1.00-10.0 µg/mL.

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human CD43 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human CD43 binds to Recombinant Human Siglec‑1/CD169 (Catalog # 5197-SL) with an ED₅₀ of 1.00-10.0 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human CD43 protein
  Ser20-Arg253, with a C-terminal 10-His tag
• Accession #: P16150
• N-terminal Sequence: Ser20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 77-88 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD43 Protein, CF

• CD43 antigen
• CD43
• CD43)
• Galactoglycoprotein
• GALGP
• GPL115
• Leukocyte sialoglycoprotein
• Leukosialin
• LSN
• Ly-48
• sialophorin (gpL115, leukosialin, CD43)
• Sialophorin
• SPN

Background

CD43, also known as Leukosialin and Sialophorin and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion (3). In human, CD43 is synthesized as a 400 amino acid (aa) precursor that contains a 19 aa signal sequence, a 234 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. The extracellular region (aa 20-253) has 25 O-linked glycosylation sites and 1 N-linked glycosylation site. Human CD43 extracellular region has less than 60% aa identity to the extracellular regions in rat and mouse.

1. Santamaría, M. et al. (1996). Cancer Res. 56:3526.
2. Manjunath, N. et al. (1995) Nature 377:535.
3. Brown, T. J. et al. (1996) J. Biol. Chem. 271:27686.

Publications for CD43/Sialophorin (9680-CD)(2)

Publications using 9680-CD

• BJ Medley, L Leclaire, N Thompson, KE Mahoney, B Pluvinage, MAH Parson, JE Burke, S Malaker, W Wakarchuk, AB Boraston A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond The Journal of Biological Chemistry, 2022-08-30;0(0):102439. 2022-08-30 [PMID: 36049519] (Bioassay, Human)
• DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences The Journal of Biological Chemistry, 2022-04-09;0(0):101917. 2022-04-09 [PMID: 35405095] (Bioassay, Akkermansia muciniphila)

Bioinformatics

• Uniprot:
  • P16150()