Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect 0.6-6.0 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human CD300e/LMIR6 protein
  

  Human CD300e/LMIR6 (Leu18-Leu169) Accession # Q496F6.2	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q496F6.2
• N-terminal Sequence: Leu18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-58 kDa and 106-125 kDa (non-reducible dimer), under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

• CD300 antigen like family member E
• CD300 antigen-like family member E
• CD300e antigenPIgR-2
• CD300e molecule
• CD300e
• CD300LE
• CD300LEPIgR2
• CLM2
• CLM-2
• CLM2CMRF35-A5
• CMRF35A5
• CMRF35-like molecule 2
• Immune receptor expressed on myeloid cells 2
• IREM-2
• IREM2IREM-2
• LMIR6
• Poly-Ig receptor 2
• Polymeric immunoglobulin receptor 2

Background

CD300e, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35-kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 156 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 11 aa cytoplasmic stub (2). The ECD contains an Ig-like V-type domain with a single disulfide bond and an N-linked glycosylation site. Within the ECD, human CD300e shares 57% sequence identity with mouse and rat CD300e. Its transmembrane segment contains a charged lysine residue which mediates CD300e association with the signaling adaptor molecule DAP12 and enables CD300e to function as an activating receptor (2, 3, 5). The ligand of CD300e is unknown; sphingomyelin has been demonstrated to bind human and mouse CD300e (5). CD300e is highly expressed on intermediate and non-classical monocytes, and myeloid dendritic cells (3, 4). CD300e on monocytes from HIV-infected patients under cART is correlated with markers of disease progression and immune inflammation (6). The cross-linking of CD300e enhances LPS-mediated cytokine production by monocytes, enhancing TNF-alpha production while no enhancement is observed on IL-1 beta, IL-6 and IL-10 (7). Our in-house data indicate that CD300e inhibits T cell activation, including anti-CD3-induced IL-2 and IFN-gamma secretion.

1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.
3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.
4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.
5. Isobe, M. et al. (2018) J. Biol. Chem. 293:3793.
6. Vitalle, J. et al. (2017) Front. Immunol. 8:836.
7. Zenarruzabeitia O. et al. (2016) Sci Rep. 6:32693.

Bioinformatics

• Uniprot:
  • Q496F6.2()