Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF

When Recombinant Human Siglec-10 Fc Chimera (2130-SL) is coated at 5 μg/mL, the concentration of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag (Catalog # AVI5247) that produces 50% optimal binding response is found to be approximately 1.5-7.5 μg/mL.

2 μg/lane of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag Protein (Catalog # AVI5247) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-55 kDa and 80-100 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) is coated at 5 μg/mL, the concentration of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag (Catalog # AVI5247) that produces 50% optimal binding response is found to be approximately 1.5‑7.5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD24 protein
  

  Human CD24 (Ser27-Gly59) Accession # P25063.3	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: P25063.3
• N-terminal Sequence: Ser27
• Structure / Form: Disulfide-linked homodimer, biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 43-55 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF

• CD 24
• CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• CD24 antigen
• CD24 molecule
• CD24
• CD24A
• CD24Asignal transducer CD24
• FLJ22950
• FLJ43543
• MGC75043
• Small cell lung carcinoma cluster 4 antigen

Background

CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa-60 kDa GPI-linked sialoprotein (1-4). Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells (4-6). In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells (2). CD24 expression is regulated during lineage development and with the activation of various cell types (4). Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance (7-11). CD24 on antigen presenting cells cooperates with B7 molecules in the co-stimulation of T cells (12-14). CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells (15). Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs (15, 16). Mature human CD24 shares 30% and 42% amino acid sequence identity with mouse and rat CD24, respectively. Our Avi-tag Biotinylated human CD24 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Jackson, D. et al. (1992) Cancer Res. 52:5264.
2. Kay, R. et al. (1991) J. Immunol. 147:1412.
3. Kadmon, G. et al. (1992) J. Cell Biol. 118:1245.
4. Fang, X. et al. (2010) Cell. Mol. Immunol. 7:100.
5. Fischer, G. F. et al. (1990) J. Immunol. 144:638.
6. Allman, D.M. et al. (1993) J. Immunol. 151:4431.
7. Suzuki, T. et al. (2001) J. Immunol. 166:5567.
8. Taguchi, T. et al. (2003) J. Immunol. 170:252.
9. Jung, K.C. et al. (2004) J. Immunol. 172:795.
10. Kishimoto, H. and J. Sprent (1997) J. Exp. Med. 185:263.
11. Hitsumoto, Y. et al. (1996) Immunology 89:200.
12. Liu, Y. et al. (1992) Eur. J. Immunol. 22:2855.
13. Enk, A.H. and S.I. Katz (1994) J. Immunol. 152:3264.
14. Askew, D. and C.V. Harding (2008) Immunology 123:447.
15. Chen G.Y. et al. (2009) Science 323:1722.
16. Liu, Y. et al. (2011) Curr. Opin. Immunol. 23:41.

Bioinformatics

• Uniprot:
  • P25063.3()