Recombinant Human CCL26/Eotaxin-3 (aa 24-94) Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CCL26/Eotaxin-3 (aa 24-94) Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CCR3. The ED 50 for this effect is 80.0-800 ng/mL.
• Source: E. coli-derived human CCL26/Eotaxin-3 protein Thr24-Leu94
• Accession #: Q9Y258.1
• N-terminal Sequence: Thr24
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL26
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL26/Eotaxin-3 (aa 24-94) Protein, CF

• CC chemokine IMAC
• C-C motif chemokine 26
• CCL26
• chemokine (C-C motif) ligand 26
• chemokine N1
• Eotaxin3
• Eotaxin-3
• IMAC
• Macrophage inflammatory protein 4-alpha
• MGC126714
• MIP-4a
• MIP-4alpha
• SCYA26
• SCYA26MIP-4-alpha
• small inducible cytokine A26
• small inducible cytokine subfamily A (Cys-Cys), member 26
• Small-inducible cytokine A26
• TSC-1Thymic stroma chemokine-1

Background

Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4. Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Recombinant Eotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.

1. Gou, R.F. et al. (1999) Genomics 58:313.
2. Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
3. Shinkai, A. et al. (1999) J. Immunol. 163:1602.

Publications for CCL26/Eotaxin-3 (346-E3/CF)(2)

Publications using 346-E3/CF

• Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. Blood, 2004-03-25;104(2):314-20. 2004-03-25 [PMID: 15044249] (ELISA (Standard))
• Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding. Eur. J. Pharmacol., 2002-12-05;456(1):1-10. 2002-12-05 [PMID: 12450563] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CCL26
• Uniprot:
  • Q9Y258.1()