Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein Summary

Details of Functionality
Measured by its ability to chemoattract Y3 rat myeloid cells transfected with human CCR3. The ED50 for this effect is 0.01-0.05 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 0.01-0.05 µg/mL.
Source
E. coli-derived human CCL24/Eotaxin-2/MPIF-2 protein
Val27-Cys119
Accession #
N-terminal Sequence
Val27
Protein/Peptide Type
Recombinant Proteins
Gene
CCL24
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
343-E2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein

  • CCL24
  • chemokine (C-C motif) ligand 24
  • Ckb-6
  • Eosinophil chemotactic protein 2
  • Eotaxin-2
  • member 24
  • MPIF2
  • MPIF-2
  • MPIF2Myeloid progenitor inhibitory factor 2
  • MPIF-2Small-inducible cytokine A24
  • SCYA24CK-beta-6

Background

Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF-2/Eotaxin-2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy-terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42% and 39% amino acid identity to MCP-3, MIP-1 alpha, and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes.

Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR-3, at least on eosinophils.

  1. Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
  2. Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.

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Publications for CCL24/Eotaxin-2/MPIF-2 (343-E2)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(7)
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Human
(7)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 343-E2 Applications Species
Dyer, Kimberly, Rosenberg, Helene F Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24). PLoS ONE, 2015;10(3):e0118887. 2015 [PMID: 25774667] (Bioassay, Human) Bioassay Human
Sturm E, Dyer K, Percopo C, Heinemann A, Rosenberg H Chemotaxis of bone marrow derived eosinophils in vivo: a novel method to explore receptor-dependent trafficking in the mouse. Eur J Immunol, 2013;43(8):2217-28. 2013 [PMID: 23670593] (Mouse) Mouse
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O&amp;amp;apos;Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma. J. Immunol., 2007;178(12):7879-89. 2007 [PMID: 17548626] (Bioassay, Human) Bioassay Human
Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C Functional analysis of the chemokine receptor CCR3 on airway epithelial cells. J. Immunol., 2006;177(5):3344-54. 2006 [PMID: 16920975] (Bioassay, Human) Bioassay Human
Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands. J. Immunol., 2006;176(10):5760-71. 2006 [PMID: 16670281] (Bioassay, Human) Bioassay Human
Yamamoto H, Nagata M, Sakamoto Y CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005;94(2):292-300. 2005 [PMID: 15765748] (Bioassay, Human) Bioassay Human
Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding. Eur. J. Pharmacol., 2002;456(1):1-10. 2002 [PMID: 12450563] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol CCL24
Uniprot