Recombinant Human CCL2/MCP-1 (Mucin Stalk Chimera), CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CCL2/MCP-1 (Mucin Stalk Chimera), CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2. The ED₅₀ for this effect is 0.20-0.60 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived human CCL2/JE/MCP-1 protein
  

  Human CCL2 (Gln24 - Thr99) Accession # P13500	GS	Human Fractalkine mucin-like stalk (Phe103 - Gln341) Accession # P78423	KQN	6-His tag
  N-terminus				C-terminus

• Accession #: P13500 (CCL2) & P78423 (CX3CL1 Mucin-like Stalk)
• N-terminal Sequence: No results obtained: Gln24 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL2/MCP-1 (Mucin Stalk Chimera), CF

• C-C motif chemokine ligand 2
• CCL2
• GDCF-2
• HC11
• HSMCR30
• MCAF
• Mcp1
• MCP-1
• SCYA2
• SMC-CF

Background

Fractalkine (also known as neurotactin and CX₃CL1) is one of two known transmembrane chemokines and is the only member of the CX₃C chemokine subfamily. Fractalkine is a type I membrane protein containing a chemokine domain tethered on a long mucin-like stalk that is connected to a transmembrane domain and a C-terminal intracellular domain. Expression of Fractalkine has been detected on endothelial cells, neurons, dendritic cells and a subset of mononuclear phagocytes. The expression of Fractalkine is upregulated in response to inflammatory signals. Fractalkine binds with high affinity to CX₃CR1 and mediates the tight binding of leukocytes expressing CX₃CR1 independently of G protein activation under flow conditions. In the MCP-1/mucin-like stalk chimera, the chemokine domain of human Fractalkine is replaced by human MCP-1 (CCL2), a ligand for CCR2.

1. Haskell, C.A. et al. (2000) J. Biol. Chem. 275:34183.

Bioinformatics

• Gene Symbol: CCL2
• Uniprot:
  • P13500 (CCL2) & P78423 (CX3CL1 Mucin-like Stalk)()