Recombinant Human CCL14a/HCC-1 (aa 28-93) Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CCL14a/HCC-1 (aa 28-93) Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.15-0.75 ng/mL.
• Source: E. coli-derived human CCL14a/HCC-1 protein
  

  M	Human CCL14a/HCC-1 (Gly28-Asn93) Accession # Q16627	WIGDPS
  N-terminus		C-terminus

• Accession #: Q16627
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL14
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 7.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL14a/HCC-1 (aa 28-93) Protein, CF

• CCL14
• CCL14a
• HCC1
• HCC-1

Background

HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue precursor with a 19 aa signal peptide that is cleaved to form the 74 aa mature secreted proprotein. By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa mature propeptide is a weak CCR1 agonist, the proteolytically processed truncated HCC-1/CCL14a (aa 28 - 93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28 - 93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a propeptide to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5).

1. Schulz-Knappe, P. et al. (1996) J. Exp. Med. 183:295.
2. Forssmanns, U. et al. (2001) J. Leukocyte Biology 70:357.
3. Richter, R. et al. (2000) Biochemistry 39:10799.
4. Munch, J. et al. (2002) Antimicrob. Agents Chemother. 46:982.
5. Vakili, J. et al. (2001) J. Immunol. 167:3406.

Publications for CCL14a/HCC-1 (1578-HC/CF)(1)

Publication using 1578-HC/CF

• R Sakumoto, KG Hayashi, S Fujii, H Kanahara, M Hosoe, T Furusawa, K Kizaki Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy Int J Mol Sci, 2017;18(4):. 2017 [PMID: 28362325] (Bioassay, Bovine)

Bioinformatics

• Gene Symbol: CCL14
• Uniprot:
  • Q16627()