Details of Functionality | Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.15-0.75 ng/mL. |
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Source | E. coli-derived human CCL14a/HCC-1 protein
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Accession # | |||||||
N-terminal Sequence | Met |
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Protein/Peptide Type | Recombinant Proteins |
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Gene | CCL14 |
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Purity | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
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Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 7.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue precursor with a 19 aa signal peptide that is cleaved to form the 74 aa mature secreted proprotein. By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa mature propeptide is a weak CCR1 agonist, the proteolytically processed truncated HCC-1/CCL14a (aa 28 - 93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28 - 93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a propeptide to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5).
Publication using 1578-HC/CF | Applications | Species |
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R Sakumoto, KG Hayashi, S Fujii, H Kanahara, M Hosoe, T Furusawa, K Kizaki Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy Int J Mol Sci, 2017-03-31;18(4):. 2017-03-31 [PMID: 28362325] (Bioassay, Bovine) | Bioassay | Bovine |
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