Recombinant Human BMP-4/BMP-7 Heterodimer Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-4/BMP-7 Heterodimer Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 15-75 ng/mL.
Source
E. coli-derived human BMP-4/BMP-7 Heterodimer protein
Met Human BMP-4
(Ser293-Arg408)
Accession # P12644
Human BMP-7
(Ser293-His431)
Accession # P18075
N-terminus C-terminus
Accession #
N-terminal Sequence
Met (BMP-4) & Ser293 (BMP-7)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.3 kDa (BMP-4), 15.8 kDa (BMP-7).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
3727-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-4/BMP-7 Heterodimer Protein

  • BMP-4/BMP-7 Heterodimer

Background

Human Bone Morphogenetic Protein 4 and 7 (BMP-4 and BMP-7), also known as osteogenic protein 1 (OP-1), belong to distinct BMP subgroups of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-4 and BMP-7 are widely expressed from early embryogenesis to adult and influence a variety of morphogenic processes (1-3). The human BMP-4 cDNA encodes a 408 amino acid (aa) precursor that includes a 19 aa signal sequence, a 273 aa propeptide, and a 116 aa mature protein (4). The human BMP-7 cDNA encodes a 431 aa precursor that includes a 29 aa signal sequence, a 263 aa propeptide, and a 139 aa mature protein (5). BMP propeptides are removed intracellulaly by proteolysis, enabling mature BMPs to form active disulfide linked homodimers or heterodimers (1). Cell types that express both BMP components can produce the heterodimers. BMP-4 and BMP-7 are each 98% conserved between human and mouse. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60 - 70% aa sequence identity with BMP-5, -6, and -8 and less than 50% aa sequence identity with other BMPs. BMP‑4 and BMP‑7, when associated into 36 kDa heterodimers (6, 7), acquire increased potency in in vitro osteogenic differentiation assays and in vivo bone formation models compared to either homodimer (6-10). Furthermore, BMP-4/BMP-7 heterodimers induce ventral mesoderm and blood formation in mid-blastula Xenopus animal cap assays, whereas the homodimers do not (11, 12). BMP-4/BMP-7 as well as either homodimer can modulate mesoderm induction by activin and prevent subsequent dorsalization of the mesoderm (12).

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Oida, S. et al. (1995) DNA Seq. 5:273.
  5. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  6. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  7. Hazama, M. et al. (1995) Biochem. Biophys. Res. Commun. 209:859.
  8. Kusumoto, K. et al. (1997) Biochem. Biophys. Res. Commun. 239:575.
  9. Zhao, M. et al. (2005) J. Cell Biochem. 95:1.
  10. Israel, D.I. et al. (1996) Growth Factors 13:291.
  11. Suzuki, A. et al. (1997) Biochem. Biophys. Res. Commun. 232:153.
  12. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.

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3727-BP
Species: Hu
Applications: Bioactivity

Publications for BMP-4/BMP-7 Heterodimer (3727-BP)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Xenopus, Zebrafish.

We have publications tested in 4 applications: Bioassay, In vivo injection, Surface Plasmon Resonance, WB Standard.


Filter By Application
Bioassay
(6)
In vivo injection
(1)
Surface Plasmon Resonance
(1)
WB Standard
(1)
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Human
(5)
Mouse
(1)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 3727-BP Applications Species
I Wagner, H Wang, PM Weissert, WL Straube, A Shevchenko, M Gentzel, G Brito, A Tazaki, C Oliveira, T Sugiura, A Shevchenko, A Simon, DN Drechsel, EM Tanaka Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration Dev. Cell, 2017;40(6):608-617.e6. 2017 [PMID: 28350991] (WB Standard) WB Standard
Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 28028233] (Bioassay, Human) Bioassay Human
Differential expression of TGF-? superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation Sci Rep, 2016;6(0):36655. 2016 [PMID: 27848974] (Bioassay, Human) Bioassay Human
A strategy to ensure safety of stem cell-derived retinal pigment epithelium cells Stem Cell Res Ther, 2016;7(1):127. 2016 [PMID: 27590276] (Bioassay, Human) Bioassay Human
Choudhary P, Dodsworth B, Sidders B, Gutteridge A, Michaelides C, Duckworth J, Whiting P, Benn C A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells. PLoS ONE, 2015;10(6):e0130379. 2015 [PMID: 26121260] (Bioassay, Human) Bioassay Human
Alexander C, Piloto S, Le Pabic P, Schilling T Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genet, 2014;10(7):e1004479. 2014 [PMID: 25058015] (In vivo injection, Zebrafish) In vivo injection Zebrafish
Lewis, Christop, Mardaryev, Andrei N, Poterlowicz, Krzyszto, Sharova, Tatyana, Aziz, Ahmar, Sharpe, David T, Botchkareva, Natalia, Sharov, Andrey A Bone morphogenetic protein signaling suppresses wound-induced skin repair by inhibiting keratinocyte proliferation and migration. J Invest Dermatol, 2014;134(3):827-37. 2014 [PMID: 24126843] (Bioassay, Mouse) Bioassay Mouse
Swapna, Immani, Borodinsky, Laura N Interplay between electrical activity and bone morphogenetic protein signaling regulates spinal neuron differentiation. Proc Natl Acad Sci U S A, 2012;109(40):16336-41. 2012 [PMID: 22991474] (Bioassay, Xenopus) Bioassay Xenopus
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011;286(34):30034-46. 2011 [PMID: 21737454] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human

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Bioinformatics

Gene Symbol BMP4
Entrez
Uniprot