Recombinant Human BLAME/SLAMF8 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human BLAME/SLAMF8 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated rhBLAME in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived human BLAME/SLAMF8 protein Ala23-Asp233, with a C-terminal 6-His tag
• Accession #: NP_064510
• N-terminal Sequence: Ala23
• Protein/Peptide Type: Recombinant Proteins
• Gene: SLAMF8
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 24.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30 - 40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BLAME/SLAMF8 Protein, CF

• B Lymphocyte Activator Macrophage Expressed
• BCM-Like Membrane Protein
• BLAME
• B-Lymphocyte Activator Macrophage Expressed
• CD353 Antigen
• CD353
• SBBI42
• SLAM Family Member 8
• SLAMF8

Background

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19% - 26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

Bioinformatics

• Gene Symbol: SLAMF8
• Uniprot:
  • NP_064510()