FCRL2/FcRH2 Antibody (296902) [Unconjugated]

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Human peripheral blood monocytes (PBMC) were labeled with Mouse Anti-Human FCRL2/FcRH2 Monoclonal Antibody (Catalog # MAB2048) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
296902
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

FCRL2/FcRH2 Antibody (296902) [Unconjugated] Summary

Additional Information
Clone 296902 was used by HLDA to establish CD designation
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FCRL2/FcRH2
Glu15-Asp395
Accession # Q96LA5
Specificity
Detects human FCRL2/FcRH2 and FCRL5/FcRH5 in Western blots.  In Western blots, approximately 25% cross-reactivity with recombinant human FCRL1, FCRL3, and FCRL4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FCRL2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2048 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRL2/FcRH2 Antibody (296902) [Unconjugated]

  • CD307b antigen
  • CD307b
  • Fc receptor homolog 2
  • Fc receptor-like 2
  • FcRH2
  • FCRH2SPAP1B
  • FCRL2
  • FcR-like protein 2
  • IFGP family protein 4
  • IFGP4
  • Immunoglobulin receptor translocation-associated protein 4
  • immunoglobulin superfamily Fc receptor, gp42
  • IRTA4
  • IRTA4SPAP1C
  • SH2 domain containing phosphatase anchor protein 1
  • SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2
  • SPAP1
  • SPAP1A
  • SPAP1immune receptor translocation-associated protein 4

Background

Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3‑5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27+ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).

 

  1. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  2. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
  3. Miller, I. et al. (2002) Blood 99:2662.
  4. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  5. Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
  6. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  7. Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
  8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  9. Huttmann, A. et al. (2006) Leukemia 20:1774.
  10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
  11. Li, F.J. et al. (2008) Blood 112:179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FCRL2/FcRH2 Antibody (MAB2048)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol FCRL2
Uniprot