Recombinant Human B7-H6 Fc Chimera Protein, CF

Recombinant Human B7-H6 Fc Chimera (Catalog #7144-B7) induces IFN-gamma secretion by NK‑92human natural killer lymphoma cells. The ED50 for this effect is0.6‑3 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human B7-H6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to induce IFN-gamma secretion by NK‑92 human natural killer lymphoma cells. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495. The ED₅₀ for this effect is 0.6-3 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human B7-H6 protein
  

  Human B7-H6 (Met1-Ser262) Accession # NP_001189368	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001189368
• N-terminal Sequence: Asp25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: NCR3LG1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 53.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 75-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human B7-H6 Fc Chimera Protein, CF

• B7 homolog 6
• B7H6
• B7-H6
• DKFZp686I21167
• DKFZp686O24166
• NCR3LG1

Background

B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).

1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.

Publications for B7-H6 (7144-B7)(1)

Publication using 7144-B7

• Maryam Salimi, Luzheng Xue, Helen Jolin, Clare Hardman, David J. Cousins, Andrew N. J. McKenzie et al. Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production1 The Journal of Immunology 2016-01-01 [PMID: 26582946] (Bioassay, Human)

Bioinformatics

• Gene Symbol: NCR3LG1
• Uniprot:
  • NP_001189368()