B7-H7/HHLA2 Antibody (907812) [Unconjugated]

Images

 
HEK293 human embryonic kidney cell line transfected with either (A) human B7-H7/HHLA2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) ...read more
In a functional flow cytometry test, biotinylated recombinant human TMIGD2/CD28H (10 ng/mL, Catalog # 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/HHLA2 (black dotted line). ...read more
In a Surface Plasmon Resonance test, Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR), coated at 10 ng/mL binds to Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7, 5 μg/mL) (blue line). Binding ...read more
B7-H7/HHLA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) at 25 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ICC/IF
Clone
907812
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

B7-H7/HHLA2 Antibody (907812) [Unconjugated] Summary

Immunogen
Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2
Met1-Asn344
Accession # Q9UM44
Specificity
Detects human B7-H7/HHLA2 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HHLA2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction 100-300 ng/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H7/HHLA2 Antibody (907812) [Unconjugated]

  • B7H7
  • B7-H7
  • HERV-H LTR-associating 2
  • HERV-H LTR-associating protein 2
  • HHLA2
  • Human endogenous retrovirus-H long terminal repeat-associating protein 2

Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5).
B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma , TNF-alpha , IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

1849-NK
Species: Hu
Applications: BA
NB500-317
Species: Bv(-), Ca(-), Ch(-), Hu, Mu(-), Rb(-)
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA, WB
NBP2-25255
Species: Hu, Mu
Applications: Flow, IHC, IHC-P, WB
NB120-6405
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MAB7144
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, KO
NB100-1793
Species: Hu, Pm, Mu, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
NBP1-86331
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-82541
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for B7-H7/HHLA2 Antibody (MAB80841) (0)

There are no publications for B7-H7/HHLA2 Antibody (MAB80841).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for B7-H7/HHLA2 Antibody (MAB80841) (0)

There are no reviews for B7-H7/HHLA2 Antibody (MAB80841). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for B7-H7/HHLA2 Antibody (MAB80841) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB80841U
Alexa Fluor 405 FAB80841V
Alexa Fluor 488 FAB80841G
Alexa Fluor 532 FAB80841X
Alexa Fluor 594 FAB80841T
Alexa Fluor 647 FAB80841R
Alexa Fluor 647 FAB80841R
Alexa Fluor 700 FAB80841N
Alexa Fluor 750 FAB80841S
Allophycocyanin FAB80841A
Biotin FAB80841B
DyLight 350 FAB80841D
DyLight 405 FAB80841E
DyLight 488 FAB80841K
DyLight 550 FAB80841L
DyLight 594 FAB80841M
DyLight 650 FAB80841W
DyLight 680 FAB80841Y
DyLight 755 FAB80841Z
FITC FAB80841F
Janelia Fluor 549 FAB80841I
Janelia Fluor 646 FAB80841J
PE FAB80841P
PerCP FAB80841C

Additional B7-H7/HHLA2 Products

Bioinformatics Tool for B7-H7/HHLA2 Antibody (MAB80841)

Discover related pathways, diseases and genes to B7-H7/HHLA2 Antibody (MAB80841). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on B7-H7/HHLA2

There are no specific blogs for B7-H7/HHLA2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our B7-H7/HHLA2 Antibody (907812) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol HHLA2
Uniprot