Recombinant Human Apolipoprotein E R2 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Apolipoprotein E R2 Protein, CF Summary

• Details of Functionality: Measured by its ability to compete with biotinylated rhApoE R2 for binding to rmReelin (Catalog # 3820-MR) in a functional ELISA. Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Apolipoprotein E R2/ApoE R2 protein
  Asp35-Lys818 (Ala262Val), with a C-terminal 10-His tag
• Accession #: Q14114
• N-terminal Sequence: Asp35
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRP8
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 87.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 140-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Apolipoprotein E R2 Protein, CF

• ApoER2
• APOER2HSZ75190
• Apolipoprotein E R2
• Apolipoprotein E receptor 2
• low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
• low-density lipoprotein receptor-related protein 8
• Lr8b
• LRP8
• LRP-8
• MCI1

Background

ApoE R2, also known as LRP8, is an LDL receptor family protein that is involved in nervous system development and function (1, 2). Human ApoE R2 has a 794 amino acid (aa) extracellular domain (ECD) and a 116 aa cytoplasmic region. The ECD contains seven LDLR class A repeats followed by two EGF-like repeats, five LDLR class B repeats, and a region rich in O-linked glycosylation. Alternatively spliced variants of human ApoE R2 have deletions of LDLR-A repeats # 4 - 7, the O-linked glycosylation region, or approximately half of the cytoplasmic domain (3). Cleavage of ApoE R2 by gamma -secretase, metalloproteases, and furin-like enzymes results in the liberation of the cytoplasmic, extracellular, and ligand binding domains (4 - 6). The cytoplasmic NPxY motif binds to PTB domain-containing adaptor proteins (7 - 10). Among these, FE65 and PSD95 couple ApoE R2 with APP or the NMDA receptor, respectively (9, 10). ApoE R2 and NMDA receptor ligands modulate adaptor interactions, NMDA-mediated ion currents, and the proteolytic processing of both ApoE R2 and APP (9 - 12). ApoE R2 and the NMDA receptor also associate extracellularly (10). ApoE R2 binds ApoE-containing lipoproteins and the large modular protein, reelin (13). Reelin signaling through ApoE R2 is critical for neuronal migration and positioning in the developing brain (13). In the adult brain, ApoE R2 functions in hippocampal synapse maturation, synaptic plasticity, and memory formation (7, 11, 14, 15). ApoE R2 is also expressed at the neuromuscular junction but does not appear to participate in the development or function of that structure (16). Deficits in ApoE R2, reelin, and ApoE function have been linked to several cognitive and mood disorders (1). Within the ECD, human ApoE R2 shares 89% aa sequence identity with mouse and rat ApoE R2. It shares 46% and 48% aa sequence identity with human LDL R and VLDL R, respectively.

1. Qiu, S. et al. (2006) Neurobiol. Learn. Mem. 85:16.
2. May, P. et al. (2005) Cell. Mol. Life Sci. 62:2325.
3. Kim, D.H. et al. (1997) J. Biol. Chem. 272:8498.
4. May, P. et al. (2003) J. Biol. Chem. 278:37386.
5. Koch, S. et al. (2002) EMBO J. 21:5996.
6. Hoe, H.-S. and G.W. Rebeck (2005) Mol. Brain Res. 137:31.
7. Beffert, U. et al. (2006) J. Neurosci. 26:2041.
8. Sun, X.M. and A.K. Soutar (2003) J. Biol. Chem. 278:19926.
9. Hoe, H.-S. et al. (2006) J. Biol. Chem. 281:3425.
10. Beffert, U. et al. (2005) Neuron 47:567.
11. Hoe, H.-S. et al. (2005) Mol. Cell. Biol. 25:9259.
12. D’Arcangelo, G. et al. (1999) Neuron 24:471.
13. Niu, S. et al. (2004) Neuron 41:71.
14. Weeber, E.J. et al. (2002) J. Biol. Chem. 277:39944.
15. Quattrocchi, C.C. et al. (2003) Science 301:649.

Publications for Apolipoprotein E R2/ApoE R2 (3520-AR)(2)

Publications using 3520-AR

• BL Roberts, BJ Bennett, CM Bennett, JM Carroll, LS Dalbøge, C Hall, W Hassouneh, KM Heppner, MA Kirigiti, SR Lindsley, KG Tennant, CA True, A Whittle, AC Wolf, CT Roberts, M Tang-Chris, MW Sleeman, MA Cowley, KL Grove, P Kievit Reelin is modulated by diet-induced obesity and has direct actions on arcuate proopiomelanocortin neurons Mol Metab, 2019-06-08;0(0):. 2019-06-08 [PMID: 31230943] (Bioassay, Human)
• Liu M, Wu G, Baysarowich J PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult mouse brain. J. Lipid Res., 2010-05-07;51(9):2611-8. 2010-05-07 [PMID: 20453200] (Western Blot)

Bioinformatics

• Gene Symbol: LRP8
• Uniprot:
  • Q14114()