Recombinant Human Angiopoietin-like 6 Protein, CF

Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 (Catalog # 8466-AN/CF) binds Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (2078-T4) with an ED50 of <2.00 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Angiopoietin-like 6 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 (Catalog # 8466-AN/CF) binds Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) with an ED₅₀ of <2.00 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 6/ANGPTL6 protein
  Ala20-Leu470, with an N-terminal c-Myc tag
• Accession #: Q8NI99
• N-terminal Sequence: Glu (c-Myc tag)
• Structure / Form: Disulfide-linked homo-oligomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ANGPTL6
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 58-69 kDa and 31-41 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 6 Protein, CF

• AGF
• AGFAngiopoietin-related protein 5
• Angiopoietin like Protein 6
• angiopoietin-like 6
• Angiopoietin-like Protein 6
• Angiopoietin-related growth factor
• ANGPTL6
• ARP5
• ARP5angiopoietin-related protein 6

Background

Angiopoietin-like Protein 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain and a fibrinogen-like domain (1, 2). Mature human ANGPTL6 shares 75% sequence identity with both mouse and rat ANGPTL6. ANGPTL6 is highly expressed in hepatocytes and is detectable in serum (1, 2). Within the liver, secreted ANGPTL6 inhibits gluconeogenesis and promotes insulin sensitivity and energy expenditure (3, 4). Circulating ANGPTL6 promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis (5, 6). An integrin­-binding RGD motif located within the fibrinogen-like domain of ANGPTL6, enables it to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells (5, 7). Elevated levels of ANGPTL6 in serum are associated with metabolic syndrome, diabetes, and pre-eclampsia (8-11). Serum ANGPTL6 levels are also elevated during obesity (4, 12). ANGPTL6 is reported to promote the survival and expansion of hematopoietic stem cells (13). ANGPTL6 is produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells (7).

1. Oike, Y. and M. Tabata (2009) Circ. J. 73:2192.
2. Kadomatsu, T. et al. (2011) FEBS J. 278:559.
3. Kitazawa, M. et al. (2007) J. Pharmacol. Exp. Ther. 323:787.
4. Oike, Y. et al. (2005) Nat. Med. 11:400.
5. Oike, Y. et al. (2003) Proc. Natl. Acad. Sci. U S A 100:9494.
6. Okazaki, H. et al. (2012) J. Dermatol. 39:366.
7. Zhang, Y. et al. (2006) Biochem. Biophys. Res. Commun. 347:100.
8. Legry, V. et al. (2009) Diabetes. Metab. 35:287.
9. Namkung, J. et al. (2011) Metabolism 60:564.
10. Stepan, H. et al. (2009) Am. J. Hypertens. 22:314.
11. Ebert, T. et al. (2009) Metabolism 58:547.
12. Ebert, T. et al. (2014) Horm. Metab. Res. 46:685.
13. Akhter, S. et al. (2013) Protein Cell 4:220.

Bioinformatics

• Gene Symbol: ANGPTL6
• Uniprot:
  • Q8NI99()