Recombinant Human ALK/CD246 His-tag Protein, CF

In a Human ALK/CD246 Antibody (AF4210) coated plate, when Recombinant Human ALK/CD246 (Catalog # 4210-CD) is present at 0.5 μg/mL, Recombinant Human Pleiotrophin/PTN (252-PL) binds with an ED50 of 20-100 ng/mL.

2 μg/lane of Recombinant Human ALK.CD246 His-tag (Catalog # 4210-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ALK/CD246 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. In a Human ALK/CD246 Antibody (Catalog # AF4210) coated plate, when Recombinant Human ALK/CD246 is present at 0.5 μg/mL, Recombinant Human Pleiotrophin/PTN (Catalog # 252-PL) binds with an ED₅₀ of 20-100 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human ALK/CD246 protein
  Val19-Ser1038, with a C-terminal 6-His tag
• Accession #: Q9UM73.3
• N-terminal Sequence: Val19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 111 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-150 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ALK/CD246 His-tag Protein, CF

• ALK tyrosine kinase receptor
• ALK
• anaplastic lymphoma kinase (Ki-1)
• Anaplastic lymphoma kinase
• anaplastic lymphoma receptor tyrosine kinase
• CD246 antigen
• CD246
• EC 2.7.10.1
• Ki-1
• NBLST3
• Tcrz

Background

Anaplastic Lymphoma Kinase (ALK), also known as CD246, is a transmembrane receptor tyrosine kinase in the Insulin Receptor family. The ALK gene is a target of multiple chromosomal translocations in cancer that encode hybrid proteins which promote cellular transformation (1, 2). Human ALK consists of a 1020 amino acid (aa) extracellular domain (ECD) with two MAM domains that flank an LDLR class A domain, a 21 aa transmembrane segment, and a 561 aa cytoplasmic domain that contains the tyrosine kinase domain (3, 4). Within the ECD, human ALK shares 89% aa sequence identity with mouse and rat ALK. It is primarily expressed in the developing nervous system but is also found in various non‑neural tissues (3 ‑ 6). Mature ALK is expressed on the cell surface as a 200 ‑ 220 kDa N‑glycosylated protein (3, 4, 7). Proteolytic cleavage of ALK liberates an 80 kDa soluble fragment from the ECD with a 140 kDa fragment remaining cell‑associated (8, 9). ALK is classified as a dependence receptor, a protein that promotes apoptosis in the absence of ligand but is anti‑apoptotic upon stimulation (2, 7). Its cytoplasmic domain is cleaved following Asp1160 by Caspases during apoptosis (7). ALK stimulation by antibody ligation induces activation of its kinase domain and receptor phosphorylation, enabling the association of ALK with signal transduction proteins (4, 9). ALK binds the cytokines Pleiotrophin and Midkine, although their effects on cellular responses are not consistent between different systems (8, 10 ‑ 12). ALK promotes neurite formation in neuroblastoma cells and mediates the neuroprotective effects of Pleiotrophin in motor neurons (9, 12). A t(2;5) chromosomal translocation with Nucleophosmin (NPM) in human anaplastic large cell lymphoma results in a hybrid protein consisting of NPM fused to the kinase domain of ALK (6, 13). NPM‑ALK as well as other ALK fusion proteins are aberrantly localized to the cytoplasm and nucleus, have constitutively active kinase domains, and promote cellular transformation (1, 13, 14).

1. Chiarle, R. et al. (2008) Nat. Rev. Cancer 8:11.
2. Allouche, M. (2007) Cell Cycle 6:1533.
3. Iwahara, T. et al. (1997) Oncogene 14:439.
4. Morris, S.W. et al. (1997) Oncogene 14:2175.
5. Vernersson, E. et al. (2006) Gene Expr. Patterns 6:448.
6. Morris, S.W. et al. (1994) Science 263:1281.
7. Mourali, J. et al. (2006) Mol. Cell. Biol. 26:6209.
8. Moog-Lutz, C. et al. (2005) J. Biol. Chem. 280:26039.
9. Motegi, A. et al. (2004) J. Cell Sci. 117:3319.
10. Stoica, G.E. et al. (2001) J. Biol. Chem. 276:16772.
11. Stoica, G.E. et al. (2002) J. Biol. Chem. 277:35990.
12. Mi, R. et al. (2007) Proc. Natl. Acad. Sci. 104:4664.
13. Fujimoto, J. et al. (1996) Proc. Natl. Acad. Sci. 93:4181.
14. Bischof, D. et al. (1997) Mol. Cell. Biol. 17:2312.

Bioinformatics

• Uniprot:
  • Q9UM73.3()