Recombinant Guinea Pig IL-1 beta/IL-1F2 Protein

• Reactivity: Gp
• Applications: Bioactivity

Recombinant Guinea Pig IL-1 beta/IL-1F2 Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.

  The ED₅₀ for this effect is 7‑35 pg/mL.

• Source: E. coli-derived guinea pig IL-1 beta/IL-1F2 protein
  Thr115-Ser266, with an N-terminal Met
• Accession #: Q9WVG1.1
• N-terminal Sequence: Met
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il1b
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT, and EDTA with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Guinea Pig IL-1 beta/IL-1F2 Protein

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 18% amino acid (aa) identity in guinea pig. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 R1 binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL‑1 R3/IL‑1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 R2 has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL‑1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL‑1 R1 (1 ‑ 4). The guinea pig IL-1 beta cDNA encodes a 266 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature guinea pig IL-1 beta shares 64% ‑ 80% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus IL‑1 beta .

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
5. Accession # Q9WVG1.
6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Bioinformatics

• Gene Symbol: Il1b
• Uniprot:
  • Q9WVG1.1()