Recombinant Feline IL-2 (Cys146Ser) Protein, CF

• Reactivity: Fe
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Feline IL-2 (Cys146Ser) Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.02-0.12 ng/mL.
• Source: E. coli-derived feline IL-2 protein
  Ala21-Thr154 (Cys146Ser), with and without an N-terminal Met
• Accession #: Q07885
• N-terminal Sequence: Met & Ala21
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Feline IL-2 (Cys146Ser) Protein, CF

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin-2 (IL-2) is a secreted, single chain alpha ‑helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be O-glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4⁺ and CD8⁺ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha , beta , gamma ) in two combinations. The high‑affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha , beta , gamma  subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta  and gamma  subunits. The non-ligand binding   gamma  subunit, referred to as the common gamma  subunit ( gamma _c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8⁺ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8⁺ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4⁺ CD25⁺ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1 - 9).

1. Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038.
2. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
3. Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368.
4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
5. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
7. Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313.
8. Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443.
9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • Q07885()