Recombinant Equine IL-2 (Cys141Ser) Protein

• Reactivity: Eq
• Applications: Bioactivity

Recombinant Equine IL-2 (Cys141Ser) Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.3‑1.5 µg/mL.
• Source: E. coli-derived equine IL-2 protein
  Ala21-Thr149 (Cys141Ser), with and without an N-terminal Met
• Accession #: NP_001078902
• N-terminal Sequence: Met & Ala21
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Equine IL-2 (Cys141Ser) Protein

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). IL-2 has since been found to also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes (2).

The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor that mediate IL‑2 signals is composed of three polypeptide chains, the IL-2 receptor alpha , beta  and gamma subunits (3). IL-2 also signals via the intermediate affinity receptor complex of the beta and gamma subunits (4). In T cells, the beta  and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-21 (6).

At the amino acid sequence level, equine IL-2 shares 72%, 70%, 56% and 54% sequence similarities with human, porcine, rat and mouse IL-2, respectively. It has been reported that equine IL-2 augmented proliferation in equine peripheral blood mononuclear cells, but has no effect on mouse CTLL-2 cells (7).

1. Morgan, D.A. et al. (1976) Science 193:1007.
2. Smith, K.A. et al. (1988) Science 240:1169.
3. Taniguchi, T. and Y. Minami (1993) Cell 73:5.
4. Giri, J. et al. (1994) EMBO J. 13:2822.
5. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
6. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
7. E.V. Vandergrift and D.W. Horohov (1993) Vet. Immunol. Immunopathol. 39:395.

Publications for IL-2 (1613-IL)(1)

Publication using 1613-IL

• O Witkowska-, R Pingwara, J Szczepania, A Winnicka The Effect of the Clenbuterol-&amp;beta2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen Species Production in Race Horses In Vitro Cells, 2021-04-17;10(4):. 2021-04-17 [PMID: 33920705] (Bioassay, Equine)

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • NP_001078902()