Recombinant Equine IL-1 beta/IL-1F2 Protein


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Reactivity EqSpecies Glossary
Applications Bioactivity

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Recombinant Equine IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 5-30 pg/mL.
E. coli-derived equine IL-1 beta/IL-1F2 protein
Ala116-Ala268 (Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys and Arg256Gln)
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
3340-EL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Equine IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta


IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5 - 7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65% - 75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 48:221.
  6. Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:704.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  8. Kato, H. et al. (1996) Gene 177:11.

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Publications for IL-1 beta/IL-1F2 (3340-EL)(8)

We have publications tested in 3 confirmed species: Equine, Horse, Porcine.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

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Showing Publications 1 - 8 of 8.
Publications using 3340-EL Applications Species
L Bundgaard, A Stensballe, KJ Elbæk, LC Berg Mass spectrometric analysis of the in vitro secretome from equine bone marrow-derived mesenchymal stromal cells to assess the effect of chondrogenic differentiation on response to interleukin-1&amp;beta treatment Stem Cell Res Ther, 2020;11(1):187. 2020 [PMID: 32434555] (Cell Culture, Horse) Cell Culture Horse
L McGlinchey, G Agne, T Passler, R Cole, J Schumacher An Objective Assessment of the Effect of Anesthetizing the Median Nerve on Lameness Caused by Pain in the Cubital Joint J. Equine Vet. Sci., 2019;75(0):9-13. 2019 [PMID: 31002100] (In Vivo, Equine) In Vivo Equine
JM Cassano, LV Schnabel, MB Goodale, LA Fortier Inflammatory licensed equine MSCs are chondroprotective and exhibit enhanced immunomodulation in an inflammatory environment Stem Cell Res Ther, 2018;9(1):82. 2018 [PMID: 29615127] (Bioassay, Equine) Bioassay Equine
M Castro Mar, MJ Peffers, K Lee, LM Rubio-Mart Effects of stanozolol on normal and IL-1?-stimulated equine chondrocytes in vitro BMC Vet. Res., 2018;14(1):103. 2018 [PMID: 29554899] (Bioassay, Equine) Bioassay Equine
H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10 Virology, 2018;0(0):. 2018 [PMID: 29502802] (Bioassay, Porcine) Bioassay Porcine
LJ Berghaus, S Giguère, AI Bordin, ND Cohen Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages Cytokine, 2017;102(0):7-11. 2017 [PMID: 29245049] (Bioassay, Equine) Bioassay Equine
Danièle Noel Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFN?-Primed Equine Mesenchymal Stem Cells Front Immunol, 2016;7(0):392. 2016 [PMID: 27729913] (Bioassay, Equine) Bioassay Equine
Ley C, Svala E, Nilton A, Lindahl A, Eloranta ML, Ekman S, Skioldebrand E Effects of high mobility group box protein-1, interleukin-1beta, and interleukin-6 on cartilage matrix metabolism in three-dimensional equine chondrocyte cultures. Connect. Tissue Res., 2011;52(4):290-300. 2011 [PMID: 21117899] (Bioassay, Equine) Bioassay Equine

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IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Gene Symbol IL1B