Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein

• Reactivity: Cr
• Applications: Bioactivity

Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.15‑0.6 ng/mL.
• Source: E. coli-derived cotton rat IL-2 protein
  Ala21-Gln155 (Cys146Tyr), with and without an N-terminal Met
• Accession #: AAK94012
• N-terminal Sequence: Met & Ala21
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 (IL-2) is a protein that has a variety of immunological functions, the most notable being the ability of IL-2 to promote the proliferation and maturation of activated T cells. Other biological activities attributed to IL-2 include:

Induction of secretion of IFN-gamma and TNF-alpha and -beta from peripheral blood mononuclear cells.

Stimulation of the rate of synthesis of c-myc RNA and transferrin receptor.

Activation of neutrophils.

Stimulation of proliferation and maturation of activated helper T cells.

Stimulation of proliferation of activated and natural killer cells and tumor-infiltrating lymphocytes, as well as enhancement of the ability of these cytotoxic lymphocytes to kill target cells.

Induction of the expression of IL-2 receptors on T cells stimulation of proliferation of antibody-producing B cells.

Bioinformatics

• Uniprot:
  • AAK94012()