Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

• Reactivity: Cr
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cotton Rat CXCL10/IP-10 Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR3. The ED₅₀ for this effect is 2-10 µg/mL.
• Source: E. coli-derived cotton rat CXCL10/IP-10/CRG-2 protein
  Ile22-Val94, with an N-terminal Met
• Accession #: AAL16935
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• mob-1
• SCYB10

Background

P-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1 - 7).

1. Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.
2. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.
3. Proost, P. et al. (1993) J. Immunol. 150:1000.
4. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.
5. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.
6. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.
7. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.

Publications for CXCL10/IP-10/CRG-2 (1117-IP)(2)

Publications using 1117-IP

• Nathalie Strazielle, Rita Creidy, Christophe Malcus, José Boucraut, Jean-François Ghersi-Eg T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium. PLoS ONE, 2016-03-04;0(0):1932-6203. 2016-03-04 [PMID: 26942913] (Bioassay, Rat)
• Barkho BZ, Song H, Aimone JB Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006-06-01;15(3):407-21. 2006-06-01 [PMID: 16846377] (Bioassay, Rat)

Bioinformatics

• Uniprot:
  • AAL16935()