Recombinant Chicken Netrin-2 Protein

• Reactivity: Ch
• Applications: Binding Activity

Recombinant Chicken Netrin-2 Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rcNetrin-2 with a linear range of 6-400 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived chicken Netrin-2 protein
  Ala16-Pro581, with a C-terminal 6-His tag & Ala16-Pro581, with an N-terminal Met and C-terminal 6-His tag
• Accession #: Q90923
• N-terminal Sequence: Ala16 & Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: NTN3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Chicken Netrin-2 Protein

• Netrin2
• Netrin-2
• NTN2L

Background

Netrins are laminin-like axon guidance molecules that are conserved among C. elegans, Drosophila, and vertebrates. The chicken Netrin-2 cDNA encodes a precursor protein that has structural similarity to the N-terminus of B2 or the gamma -chain of laminin. The mature Netrin-2 protein has 581 amino acid (aa) which contains a 245 aa domain VI, a 169 aa domain V with three cysteine/glycine rich EGF repeats and a unique 152 aa domain C that enriches in basic aa residues and contains an RGD motif. Chicken Netrin-2 shares 72% aa identity with chicken Netrin-1, 51% aa identity with C. elegans UNC-6 protein and 57% aa identity with human NTN2L (Netrin‑2‑like). Although only two chicken netrins are known, the number of known mammalian netrins is growing. Mouse Netrin-3 and Netrin-4 were recently reported. Netrin-2 was expressed in the chick brain and spinal cord from E6 through to the adult. In addition, expression of Netrin-2 was observed at various stages in several mesodermally and endodermally derived tissues, including lung, gut, ovary, testes and spleen. Netrins act to both attract and repel the growing axons of a broad range of neuronal cell types during development. These actions are mediated by specific receptor complexes containing either the DCC (colorectal carcinoma) or neogenin, in the case of attractant, or UNC-5-related proteins, in the case of repellent.

1. Serafini, T. et al. (1994) Cell 78:409.
2. Kennedy, T.E. et al. (1994) Cell 78:425.
3. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.

Publications for Netrin-2 (127-N2)(1)

Publication using 127-N2

• Akino T, Han X, Nakayama H, McNeish B, Zurakowski D, Mammoto A, Klagsbrun M, Smith E Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma. Cancer Res, 2014-05-08;74(14):3716-26. 2014-05-08 [PMID: 24812271] (Western Blot)

Bioinformatics

• Gene Symbol: NTN3
• Uniprot:
  • Q90923()