Recombinant Canine IL-2 (Cys147Ser) Protein, CF

• Reactivity: Ca
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Canine IL-2 (Cys147Ser) Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.15‑0.8 ng/mL.
• Source: E. coli-derived canine IL-2 protein
  Ala21-Thr155 (Cys147Ser), with and without an N-terminal Met
• Accession #: Q29416
• N-terminal Sequence: Met & Ala21
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine IL-2 (Cys147Ser) Protein, CF

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes (2). The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha , beta , and gamma subunits (3). IL-2 binds the  alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha , beta , and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the  beta and  gamma subunits, which is also capable of transducing IL-2 signals (4). In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9 (6). At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively (7).

1. Morgan, D.A. et al. (1976) Science 193:1007.
2. Smith, K.A. et al. (1988) Science 240:1169.
3. Taniguchi, T. and Y. Minami (1993) Cell 73:5.
4. Giri, J. et al. (1994) EMBO J. 13:2822.
5. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
6. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
7. Dunham, S.P. et al. (1995) DNA Seq. 5:177.

Publications for IL-2 (1815-CL/CF)(2)

Publications using 1815-CL/CF

• BM Scorza, KG Mahachi, AD Cox, AJ Toepp, D Pessoa-Per, P Tyrrell, J Buch, JA Foltz, D Lee, CA Petersen Role of NK-Like CD8+ T Cells during Asymptomatic Borrelia burgdorferi Infection Infection and Immunity, 2022-04-13;0(0):e0055521. 2022-04-13 [PMID: 35416707] (Bioassay, Canine)
• Haurigot V, Mingozzi F, Buchlis G Safety of AAV factor IX peripheral transvenular gene delivery to muscle in hemophilia B dogs. Mol. Ther., 2010-04-27;18(7):1318-29. 2010-04-27 [PMID: 20424599] (Bioassay, Canine)

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • Q29416()