RDH16 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: RDH16 Antibody [NBP1-87117] - Analysis in human liver and pancreas tissues. Corresponding RDH16 RNA-seq data are presented for the same tissues.
Western Blot: RDH16 Antibody [NBP1-87117] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: RDH16 Antibody [NBP1-87117] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: RDH16 Antibody [NBP1-87117] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: RDH16 Antibody [NBP1-87117] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: RDH16 Antibody [NBP1-87117] - Staining of human lymph node shows no positivity as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

RDH16 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GVKVAMIEPGYFKTAVTSKERFLKSFLEIWDRSSPEVKEAYGEKFVADYKKSAEQMEQKCTQDLSLVT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RDH16
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
RDH16 Protein (NBP1-87117PEP)
Publications
Read Publication using NBP1-87117.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24648543)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RDH16 Antibody

  • EC 1.1
  • EC 1.1.1
  • EC 1.1.1.105
  • Microsomal NAD+-dependent retinol dehydrogenase 4
  • retinol dehydrogenase 16 (all-trans and 13-cis)
  • retinol dehydrogenase 16 (all-trans)
  • retinol dehydrogenase 16
  • RODH4
  • RODH-4
  • SDR9C8
  • short chain dehydrogenase/reductase family 9C, member 8
  • Sterol/retinol dehydrogenase

Background

Retinol dehydrogenase 16 (RDH16) is an oxidoreductase with a preference for NAD. It oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. RDH16 has higher activity towards CRBP-bound retinol than with free retinol. It oxidizes 3-alpha-hydroxysteroids and also oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. RDH16 can also catalyze the reverse reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RDH16 Antibody (NBP1-87117)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional RDH16 Products

Array NBP1-87117

Bioinformatics Tool for RDH16 Antibody (NBP1-87117)

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Bioinformatics

Gene Symbol RDH16
Entrez
Uniprot