RCOR1/CoREST Antibody (658526) [Alexa Fluor® 350]

• Applications: WB, ICC/IF
• Clone: 658526
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

RCOR1/CoREST Antibody (658526) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human RCOR1/CoREST
  Ala394-Ser482
  Accession # Q9UKL0
• Specificity: Detects human RCOR1/CoREST in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human RCOR3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RCOR1/CoREST Antibody (658526) [Alexa Fluor® 350]

• CoREST
• KIAA0071CORESTRCORREST corepressor
• Protein CoREST
• RCOR1
• RCOR1/CoREST
• REST corepressor 1

Background

RCOR1 (REST [RE-1 silencing transcription factor] CORepressor 1; also CoREST) is a 64-66 kDa member of the CoREST family of proteins. It is a nuclear protein that is ubiqui-tously expressed. RCOR1 serves as a molecular bridge, linking REST with Sin3, HDAC, LSD1, histone H3 K9 me-thyltransferases, and MeCP2. This creates a complex that represses the expression of neuron-specific genes in nonneuronal cells, principally by coordinating the demethylation of Lys4 in histone H3. Human RCOR1 is 482 amino acids (aa) in length. It contains one ELM2 domain (aa 102-157) that interacts with HDAC, and two SANT sequences (aa 187-238 and 378‑429) that bind DNA tandem repeats. There are three potential RCOR1 splice vari-ants. One contains a four aa substitution for aa 296-482, a second shows an alternative start site 3 aa upstream of the standard site, while a third shares the same upstream start site accompanied by a 15 aa substitution for aa 471-482. Over aa 394-482, human RCOR1 shares 93% aa identity with mouse RCOR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9UKL0(Human)