RBX1 Antibody (1076128) [mFluor Violet 450 SE]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 1076128
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

RBX1 Antibody (1076128) [mFluor Violet 450 SE] Summary

• Immunogen: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL1/RBX1 Complex
  Met1-His108
  Accession # P62877
• Specificity: Detects human RBX1 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RBX1 Antibody (1076128) [mFluor Violet 450 SE]

• BA554C12.1
• EC 6.3.2.-
• FLJ60363
• MGC13357
• Protein ZYP
• RBX1
• Regulator of cullins 1
• Ring Box 1
• RING box protein 1
• RING finger protein 75
• ring-box 1
• ring-box 1, E3 ubiquitin protein ligase
• RING-box protein 1
• RNF75
• RNF75MGC1481
• ROC1E3 ubiquitin-protein ligase RBX1
• ZYP Protein

Background

ROC1 is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.