RBP4/Retinol-Binding Protein 4 Overexpression Lysate (Native)

Images

 
Western Blot: RBP4 Overexpression Lysate (Adult Normal) [NBL1-15228] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBP4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RBP4/Retinol-Binding Protein 4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204635 and based on accession number NM_006744. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens retinol binding protein 4, plasma (RBP4), mRNA.
Gene
RBP4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RBP4/Retinol-Binding Protein 4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RBP4/Retinol-Binding Protein 4 Overexpression Lysate (Native)

  • interstitial
  • Plasma retinol-binding protein
  • RBP4
  • retinol binding protein 4, plasma
  • RetinolBinding Protein 4
  • retinol-binding protein 4, plasma

Background

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-89649
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB500-418
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
NBP2-00637
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-594
Species: Hu, Mu, Ca
Applications: WB, IHC-P
NBP1-31555
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-92164
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-81029
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP2-07799
Species: Hu
NBL1-15228
Species: Hu
Applications: WB

Publications for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228) (0)

There are no publications for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228) (0)

There are no reviews for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RBP4/Retinol-Binding Protein 4 Products

Bioinformatics Tool for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228)

Discover related pathways, diseases and genes to RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228)

Discover more about diseases related to RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228).
 

Pathways for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228)

View related products by pathway.

PTMs for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228)

Learn more about PTMs related to RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228).
 

Research Areas for RBP4/Retinol-Binding Protein 4 Lysate (NBL1-15228)

Find related products by research area.

Blogs on RBP4/Retinol-Binding Protein 4

There are no specific blogs for RBP4/Retinol-Binding Protein 4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RBP4/Retinol-Binding Protein 4 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RBP4
Entrez